Rabbit anti-Human, Mouse RBPJ Polyclonal Antibody | anti-RBPJ antibody

RBPJ (IGKJRB, IGKJRB1, RBPJK, RBPSUH, Recombining Binding Protein Suppressor of Hairless, CBF-1, J kappa-recombination Signal-binding Protein, RBP-J kappa, Renal Carcinoma Antigen NY-REN-30, MGC61669) (PE)

Cat. Num. AAA6392209

• Gene Names: RBPJ; SUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RBPJ; Polyclonal Antibody; RBPJ (IGKJRB; IGKJRB1; RBPJK; RBPSUH; Recombining Binding Protein Suppressor of Hairless; CBF-1; J kappa-recombination Signal-binding Protein; RBP-J kappa; Renal Carcinoma Antigen NY-REN-30; MGC61669) (PE); anti-RBPJ antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RBPJ. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RBPJ antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human RBPJ, aa1-487 (NP_056958.3).
• Immunogen Sequence: MAPVVTGKFGERPPPKRLTREAMRNYLKERGDQTVLILHAKVAQKSYGNEKRFFCPPPCVYLMGSGWKKKKEQMERDGCSEQESQPCAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDSDKRKHFMLSVKMFYGNSDDIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTRYLHVEGGNFHASSQQWGAFFIHLLDDDESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPRLIIRKVDKQTALLDADDPVSQLHKCAFYLKDTERMYLCLSQERIIQFQATPCPKEPNKEMINDGASWTIISTDKAEYTFYEGMGPVLAPVTPVPVVESLQLNGGGDVAMLELTGQNFTPNLRVWFGDVEAETMYRCGESMLCVVPDISAFREGWRWVRQPVQVPVTLVRNDGIIYSTSLTFTYTPEPGPRPHCSAAGAILRANSSQVPPNESNTNSEGSYTNASTNSTSVTSSTATVVS
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RBPJ rabbit polyclonal antibody. Western Blot analysis of RBPJ expression in human placenta.)

Western Blot (WB)

(RBPJ rabbit polyclonal antibody. Western Blot analysis of RBPJ expression in mouse testis.)

Western Blot (WB)

(Western Blot analysis of RBPJ expression in transfected 293T cell line by RBPJ polyclonal antibody. Lane 1: RBPJ transfected lysate (54.3kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-RBPJ antibody

Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate. determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some Notch protein, it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.

Product Categories/Family for anti-RBPJ antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 350606369
• NCBI GeneID: 3516
• NCBI Accession #: NP_056958.3
• NCBI GenBank Nucleotide #: NM_015874.3
• Molecular Weight: 54,297 Da
• NCBI Official Full Name: Homo sapiens recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: recombination signal binding protein for immunoglobulin kappa J region
• NCBI Official Symbol: RBPJ
• NCBI Official Synonym Symbols: SUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1
• NCBI Protein Information: recombining binding protein suppressor of hairless; CBF-1; H-2K binding factor-2; RBP-J kappa; RBP-JK; immunoglobulin kappa J region recombination signal binding protein 1; renal carcinoma antigen NY-REN-30; suppressor of hairless homolog
• Protein Family: RBPJ-interacting and tubulin-associated protein

NCBI Description

The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]