Rabbit RBM3 Polyclonal Antibody | anti-RBM3 antibody

RBM3 Antibody (Center)

Cat. Num. AAA9200866

• Gene Names: RBM3; RNPL; IS1-RNPL
• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RBM3; Polyclonal Antibody; RBM3 Antibody (Center); Putative RNA-binding protein 3; RNA-binding motif protein 3; RNPL; anti-RBM3 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RBM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the Central region of human RBM3.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 55-84
• Sequence Length: 157

• Applicable Applications for anti-RBM3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of RBM3 antibody (Center) in NIH-3T3 cell line lysates (35ug/lane). RBM3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(RBM3 Antibody (Center) (RB18776) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RBM3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RBM3 Antibody (Center) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of RBM3 (arrow) using rabbit polyclonal RBM3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RBM3 gene.)

Related Product Information for anti-RBM3 antibody

RBM3 is a member of the glycine-rich RNA-binding protein family and a protein with one RNA recognition motif (RRM) domain. Expression of this protein is induced by cold shock and low oxygen tension.

Product Categories/Family for anti-RBM3 antibody

Cancer; Metabolism

References

Zeng,Y., J. Cell. Biochem. 107 (1), 179-188 (2009)
Sureban,S.M., Oncogene 27 (33), 4544-4556 (2008)

NCBI and Uniprot Product Information

• NCBI GI #: 5803137
• NCBI GeneID: 5935
• NCBI Accession #: NP_006734.1
• NCBI GenBank Nucleotide #: NM_006743.4
• UniProt Accession #: P98179
• Molecular Weight: 17170
• NCBI Official Full Name: RNA-binding protein 3
• NCBI Official Synonym Full Names: RNA binding motif (RNP1, RRM) protein 3
• NCBI Official Symbol: RBM3
• NCBI Official Synonym Symbols: RNPL; IS1-RNPL
• NCBI Protein Information: RNA-binding protein 3
• UniProt Protein Name: RNA-binding protein 3
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM3
• UniProt Synonym Gene Names: RNPL
• UniProt Entry Name: RBM3_HUMAN

NCBI Description

This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2008]

Uniprot Description

RBM3: Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.

Protein type: RNA-binding; RNA processing; Ribosomal; Translation

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: cytoplasm; dendrite; nucleus; large ribosomal subunit

Molecular Function: protein binding; RNA binding; nucleotide binding; ribosomal large subunit binding

Biological Process: regulation of translation; RNA processing; miRNA-mediated gene silencing, production of miRNAs; positive regulation of translation; translation; response to cold