Testing Data
(Gel: 10%SDS-PAGE Lysate: 40ug Jurkat cell Primary antibody: 1/550 dilution Secondary antibody dilution: 1/8000 Exposure time: 30 seconds)
Rabbit anti-Human, Mouse RBFOX3 Polyclonal Antibody | anti-RBFOX3 antibody
RBFOX3 Antibody
Gene Names
RBFOX3; FOX3; NEUN; FOX-3; HRNBP3
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Synonyms
RBFOX3; Polyclonal Antibody; RBFOX3 Antibody; FOX3; NEUN; FOX-3; HRNBP3; anti-RBFOX3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total RBFOX3 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
1.1 mg/ml (varies by lot)
Sequence Length
312
Applicable Applications for anti-RBFOX3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:15-1:50
Immunohistochemistry: 1:15-1:50
Immunogen Type
Peptide
Immunogen Description
Synthetic peptide corresponding to a region derived from internal residues of human RNA binding protein, fox-1 homolog (C. elegans) 3
Target Name
RBFOX3
Preparation and Storage
Store at -20 degree C
Testing Data
(Gel: 10%SDS-PAGE Lysate: 40ug Jurkat cell Primary antibody: 1/550 dilution Secondary antibody dilution: 1/8000 Exposure time: 30 seconds)
Testing Data
(Gel: 10%SDS-PAGE Lysate: 40ug Jurkat cell Primary antibody: 1/550 dilution Secondary antibody dilution: 1/8000 Exposure time: 30 seconds)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/25.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/25.)
Related Product Information for anti-RBFOX3 antibody
Immunoprecipitation and mass spectrometry of the two major NeuN species at 45¨C50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.
Product Categories/Family for anti-RBFOX3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,123 Da
NCBI Official Full Name
RNA binding protein fox-1 homolog 3
NCBI Official Synonym Full Names
RNA binding protein, fox-1 homolog (C. elegans) 3
NCBI Official Symbol
RBFOX3
NCBI Official Synonym Symbols
FOX3; NEUN; FOX-3; HRNBP3
NCBI Protein Information
RNA binding protein fox-1 homolog 3
UniProt Protein Name
RNA binding protein fox-1 homolog 3
Protein Family
UniProt Gene Name
RBFOX3
UniProt Entry Name
RFOX3_HUMAN
Uniprot Description
NeuN: RNA-binding protein that regulates alternative splicing events.
Protein type: DNA-binding; RNA splicing; RNA processing
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: cytoplasm; perikaryon; nucleus
Molecular Function: DNA binding; RNA binding; nucleotide binding
Biological Process: RNA splicing; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome
Research Articles on RBFOX3
Similar Products
Product Notes
The RBFOX3 rbfox3 (Catalog #AAA9412383) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RBFOX3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RBFOX3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:200-1:1000 Immunohistochemistry: 1:15-1:50. Researchers should empirically determine the suitability of the RBFOX3 rbfox3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBFOX3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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