Monkey Tryptophan-2, 3-dioxygenase ELISA Kit | TDO elisa kit

Monkey Tryptophan-2, 3-dioxygenase ELISA Kit

Cat. Num. AAA748943

• Gene Names: TDO2; TO; TDO; TPH2; TRPO
• Reactivity: Monkey
• Synonyms: Tryptophan-2; 3-dioxygenase; Monkey Tryptophan-2; 3-dioxygenase ELISA Kit; TDO elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This TDO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey TDO. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TDO elisa kit

Princple of the assay: TDO ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TDO antibody and an TDO-HRP conjugate. The assay sample and buffer are incubated together with TDO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TDO concentration since TDO from samples and TDO-HRP conjugate compete for the anti-TDO antibody binding site. Since the number of sites is limited, as more sites are occupied by TDO from the sample, fewer sites are left to bind TDO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TDO concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5032165
• NCBI GeneID: 6999
• NCBI Accession #: NP_005642.1
• NCBI GenBank Nucleotide #: NM_005651.3
• Molecular Weight: 47,872 Da
• NCBI Official Full Name: tryptophan 2,3-dioxygenase
• NCBI Official Synonym Full Names: tryptophan 2,3-dioxygenase
• NCBI Official Symbol: TDO2
• NCBI Official Synonym Symbols: TO; TDO; TPH2; TRPO
• NCBI Protein Information: tryptophan 2,3-dioxygenase; tryptophanase; tryptophan oxygenase; tryptophan pyrrolase; tryptamin 2,3-dioxygenase
• UniProt Protein Name: Tryptophan 2,3-dioxygenase
• UniProt Gene Name: TDO2
• UniProt Synonym Gene Names: TDO; TDO; TO; TRPO
• UniProt Entry Name: T23O_HUMAN

NCBI Description

This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism. [provided by RefSeq, Feb 2012]

Uniprot Description

TDO2: Incorporates oxygen into the indole moiety of tryptophan. Has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin. Belongs to the tryptophan 2,3-dioxygenase family.

Protein type: Oxidoreductase; EC 1.13.11.11; Amino Acid Metabolism - tryptophan

Chromosomal Location of Human Ortholog: 4q31-q32

Cellular Component: cytosol

Molecular Function: amino acid binding; metal ion binding; heme binding; oxygen binding; tryptophan 2,3-dioxygenase activity

Biological Process: tryptophan catabolic process to acetyl-CoA; tryptophan catabolic process to kynurenine; tryptophan catabolic process