Western Blot (WB)
(RBCK1 rabbit polyclonal antibody. Western Blot analysis of RBCK1 expression in human stomach.)
Rabbit anti-Human RBCK1 Polyclonal Antibody | anti-RBCK1 antibody
RBCK1 (C20orf18, RNF54, UBCE7IP3, XAP3, XAP4, RanBP-type and C3HC4-type Zinc Finger-containing Protein 1, HBV-associated Factor 4, Heme-oxidized IRP2 Ubiquitin Ligase 1, Hepatitis B Virus X-associated Protein 4, RING Finger Protein 54, Ubiquitin-conjugati
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(RBCK1 rabbit polyclonal antibody. Western Blot analysis of RBCK1 expression in human stomach.)
Western Blot (WB)
(RBCK1 rabbit polyclonal antibody. Western Blot analysis of RBCK1 expression in human stomach.)
Western Blot (WB)
(Western Blot analysis of RBCK1 expression in transfected 293T cell line by RBCK1 polyclonal antibody. Lane 1: RBCK1 transfected lysate (25.7kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of RBCK1 expression in transfected 293T cell line by RBCK1 polyclonal antibody. Lane 1: RBCK1 transfected lysate (25.7kD). Lane 2: Non-transfected lysate.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('M-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types. Ref.2 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14
Subunit structure: Forms homodimers in vitro
By similarity. Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31. LUBAC has a MW of approximative 600 kDa suggesting a heteromultimeric assembly of its subunits. Interacts with beta-I-type (PRKCB1) and zeta-type protein kinase C (PRKCZ) and with UBE2L3. Interacts with PRKCH. Interacts with the HBV pX/HBx protein, which is required to activate transcription of the viral genome. Isoform 1 and isoform 2 interact with IREB2 only in iron-rich conditions. Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner. Interacts with EYA1, TAB2, TAB3, MAP3K7 TRAF6 and RIPK1. Interacts with IRF3. Ref.1 Ref.2 Ref.7 Ref.8 Ref.9 Ref.12 Ref.13 Ref.14 Ref.16
Induction: By viral transfection. Ref.9
Domain: The RanBP2-type zinc finger, also called Npl4 zinc finger (NZF), mediates binding to 'M-1'-linked polyubiquitins
By similarity. Ref.14The UBL domain mediates association with RNF31 via interaction with its UBA domain. Ref.14
Post-translational modification: Auto-ubiquitinated. Auto-ubiquitination leads to degradation by the proteasome
By similarity.Phosphorylated. In vitro, phosphorylation inhibits auto-ubiquitination activity
By similarity.
Sequence similarities: Contains 1 IBR-type zinc finger.Contains 1 RanBP2-type zinc finger.Contains 2 RING-type zinc fingers.Contains 1 ubiquitin-like domain.
Sequence caution: The sequence AAH15219.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on RBCK1
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Product Notes
The RBCK1 rbck1 (Catalog #AAA647759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RBCK1 (C20orf18, RNF54, UBCE7IP3, XAP3, XAP4, RanBP-type and C3HC4-type Zinc Finger-containing Protein 1, HBV-associated Factor 4, Heme-oxidized IRP2 Ubiquitin Ligase 1, Hepatitis B Virus X-associated Protein 4, RING Finger Protein 54, Ubiquitin-conjugati reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RBCK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the RBCK1 rbck1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGTATPDGRE DQERLWVSVE DAQMHTVTIW LTVRPDMTVA SLKDMVFLDY GFPPVLQQWV IGQRLARDQE TLHSHGVRQN GDSAYLYLLS ARNTSLNPQE LQRERQLRML EDLGFKDLTL QPRGPLEPGP PKPGVPQEPG RGQPDAVPEP PPVGWQCPGC TFINKPTRPG CEMCCRARPE AYQVPASYQP DEEERARLAG EEEALRQYQQ GVPAGHHPQQ PGGGGLLPLH. It is sometimes possible for the material contained within the vial of "RBCK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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