Rabbit anti-Human RASSF5 Polyclonal Antibody | anti-RASSF5 antibody

RASSF5 (Ras Association Domain-containing Protein 5, New Ras Effector 1, Regulator for Cell Adhesion and Polarization Enriched in Lymphoid Tissues, RAPL, NORE1, MGC10823, MGC17344) (PE)

Cat. Num. AAA6392099

• Gene Names: RASSF5; RAPL; Maxp1; NORE1; NORE1A; NORE1B; RASSF3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RASSF5; Polyclonal Antibody; RASSF5 (Ras Association Domain-containing Protein 5; New Ras Effector 1; Regulator for Cell Adhesion and Polarization Enriched in Lymphoid Tissues; RAPL; NORE1; MGC10823; MGC17344) (PE); anti-RASSF5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RASSF5.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RASSF5 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human RASSF5, aa1-265 (NP_872606.1).
• Immunogen Sequence: MTVDSSMSSGYCSLDEELEDCFFTAKTTFFRNAQSKHLSKNVCKPVEETQRPPTLQEIKQKIDSYNTREKNCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGIRPQSIYDAIKEVNLAATTDKRTSFYLPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDNPQKFALFKRIHKDGQVLFQKLSIADRPLYLRLLAGPDTEVLSFVLKENETGEVEWDAFSIPELQNFLTILEKEEQDKIQQVQKKYDKFRQKLEEALRESQGKPG
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of RASSF5 expression in transfected 293T cell line by RASSF5 polyclonal antibody. Lane 1: RASSF5 transfected lysate (30.4kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-RASSF5 antibody

This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-RASSF5 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 32996735
• NCBI GeneID: 83593
• NCBI Accession #: NP_872606.1
• NCBI GenBank Nucleotide #: NM_182665
• UniProt Accession #: Q8WWW0; Q5SY32; Q8WWV9; Q8WXF4; Q9BT99; A8K1E6
• Molecular Weight: 37,433 Da
• NCBI Official Full Name: ras association domain-containing protein 5 isoform C
• NCBI Official Synonym Full Names: Ras association domain family member 5
• NCBI Official Symbol: RASSF5
• NCBI Official Synonym Symbols: RAPL; Maxp1; NORE1; NORE1A; NORE1B; RASSF3
• NCBI Protein Information: ras association domain-containing protein 5
• UniProt Protein Name: Ras association domain-containing protein 5
• Protein Family: Ras association domain-containing protein
• UniProt Gene Name: RASSF5
• UniProt Synonym Gene Names: NORE1; RAPL; RAPL
• UniProt Entry Name: RASF5_HUMAN

NCBI Description

This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RASSF5: Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS1 and KRAS induced apoptosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Autophagy; Tumor suppressor; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: microtubule; cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding

Biological Process: negative regulation of cell proliferation; apoptosis; positive regulation of protein ubiquitination