EIF5A2 recombinant protein

EIF5A2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121788

• Gene Names: EIF5A2; EIF-5A2; eIF5AII
• Purity: >95% as determined by SDS-PAGE
• Synonyms: EIF5A2; EIF5A2 Protein; Human; Recombinant (His Tag); Human EIF5A2 Protein (His Tag); EIF-5A2 Protein; eIF5AII Protein; eukaryotic translation initiation factor 5A2; EIF5A2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys153

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human EIF5A2 (Q9GZV4)(Met1-Lys153) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for EIF5A2 recombinant protein

Background: Eukaryotic translation initiation factor 5A2 (EIF5A2) has been demonstrated to be upregulated in numerous types of human cancer and is associated with cancer progression. Silencing of EIF5A2 in the NSCLC cells resulted in the downregulation of the tumorigenic proteins, apoptosis regulator Bcl-2 and myc proto-oncogene protein, and upregulation of E-cadherin, suggesting that EIF5A2 promotes proliferation and metastasis through these proteins. EIF5A2 may therefore serve as a novel therapeutic target for the treatment of NSCLC. EIF5A2 might be a novel therapeutic target for the inhibition of NPC progress. EIF5A2 overexpression may contribute to cancer progression and poor prognosis, it could be a novel potential prognostic marker for FIGO stage I-II cervical cancer. EIF5A2 upregulation plays an important oncogenic role in gastric cancer. EIF5A2 may represent a new predictor for poor survival and is a potential therapeutic target for gastric cancer. The eukaryotic initiation factor 5A2 (EIF5A2) over-expression enhances HCC cell metastasis. EIF5A2, as a target of PI3K/Akt, promotes melanoma cell invasion and may serve as a promising prognostic marker and a potential therapeutic target for melanoma.

NCBI and Uniprot Product Information

• NCBI GI #: 9966867
• NCBI GeneID: 56648
• NCBI Accession #: NP_065123.1
• NCBI GenBank Nucleotide #: NM_020390.5
• UniProt Accession #: Q9GZV4; B2R4V5
• Molecular Weight: 16,793 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 5A-2
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 5A2
• NCBI Official Symbol: EIF5A2
• NCBI Official Synonym Symbols: EIF-5A2; eIF5AII
• NCBI Protein Information: eukaryotic translation initiation factor 5A-2; eIF-5A-2; eukaryotic initiation factor 5A
• UniProt Protein Name: Eukaryotic translation initiation factor 5A-2
• UniProt Gene Name: EIF5A2
• UniProt Synonym Gene Names: eIF-5A-2; eIF-5A2
• UniProt Entry Name: IF5A2_HUMAN

Uniprot Description

EIF5A2: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Belongs to the eIF-5A family.

Chromosomal Location of Human Ortholog: 3q26.2

Cellular Component: endoplasmic reticulum membrane; nuclear pore; cytosol

Molecular Function: protein binding; ribosome binding; translation elongation factor activity

Biological Process: protein transport; mRNA transport; translational frameshifting; cellular protein metabolic process; positive regulation of cell proliferation; positive regulation of translational elongation; spermatogenesis; hypusine biosynthetic process from peptidyl-lysine; polyamine homeostasis; post-translational protein modification; positive regulation of translational termination