Western Blot (WB)
((0.5ug/ml) staining of Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat RASSF3 Polyclonal Antibody | anti-RASSF3 antibody
Goat anti-RASSF3 Antibody
Gene Names
RASSF3; RASSF5
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
RASSF3; Polyclonal Antibody; Goat anti-RASSF3 Antibody; Ras association (RalGDS/AF-6) domain family 3; MGC119194; MGC119195; MGC119197; RASSF5; RASSF3 antibody; Ras association (RalGDS/AF-6) domain family 3 antibody; MGC119194 antibody; MGC119195 antibody; MGC119197 antibody; RASSF5 antibody; anti-RASSF3 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
CKPPQTSPNSGK
Sequence Length
238
Applicable Applications for anti-RASSF3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 26kDa band observed in Human Heart lysates (calculated MW of 27.6kDa according to NP_835463.1). Recommended concentration: 0.5-1.5ug/ml.
Western Blot: Approx 26kDa band observed in Human Heart lysates (calculated MW of 27.6kDa according to NP_835463.1). Recommended concentration: 0.5-1.5ug/ml.
Immunogen
Peptide with sequence CKPPQTSPNSGK, from the C Terminus of the protein sequence according to NP_835463.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.5ug/ml) staining of Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,265 Da
NCBI Official Full Name
ras association domain-containing protein 3
NCBI Official Synonym Full Names
Ras association domain family member 3
NCBI Official Symbol
RASSF3
NCBI Official Synonym Symbols
RASSF5
NCBI Protein Information
ras association domain-containing protein 3
UniProt Protein Name
Ras association domain-containing protein 3
Protein Family
UniProt Gene Name
RASSF3
UniProt Entry Name
RASF3_HUMAN
NCBI Description
The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.[supplied by OMIM, Jul 2003]
Uniprot Description
RASSF3: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 12q14.2
Cellular Component: cytoplasm; microtubule; plasma membrane
Molecular Function: identical protein binding; protein binding
Biological Process: signal transduction
Research Articles on RASSF3
Similar Products
Product Notes
The RASSF3 rassf3 (Catalog #AAA421851) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RASSF3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's RASSF3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx 26kDa band observed in Human Heart lysates (calculated MW of 27.6kDa according to NP_835463.1). Recommended concentration: 0.5-1.5ug/ml. Researchers should empirically determine the suitability of the RASSF3 rassf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CKPPQTSPNS GK. It is sometimes possible for the material contained within the vial of "RASSF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
