Rabbit anti-Human, Mouse NCALD Polyclonal Antibody | anti-NCALD antibody

NCALD, CT (NCALD, Neurocalcin-delta) (FITC)

Cat. Num. AAA6324015

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NCALD; Polyclonal Antibody; CT (NCALD; Neurocalcin-delta) (FITC); Neurocalcin-delta; anti-NCALD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NCALD antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NCALD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the C-terminal region of human NCALD.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NCALD Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the NCALD antibody detected the NCALD protein (arrow).)

Related Product Information for anti-NCALD antibody

NCALD encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.

Product Categories/Family for anti-NCALD antibody

Antibodies; Signal Transduction

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Cirulli, E.T., et al. Eur. J. Hum. Genet. 18(7):815-820(2010). Gill, M.B., et al. J. Biol. Chem. 284(21):14503-14512(2009). Venkataraman, V., et al. Biochemistry 47(25):6590-6601(2008). Kamiyama, M., et al. Hum. Genet. 122 (3-4), 397-407 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 98985785
• NCBI GeneID: 83988
• NCBI Accession #: NP_001035715.1; NP_001035720.1
• NCBI GenBank Nucleotide #: NM_001040625.1
• UniProt Accession #: P61601; P29554; Q8IYC3; Q9H0W2
• Molecular Weight: 22,245 Da
• NCBI Official Full Name: neurocalcin-delta
• NCBI Official Synonym Full Names: neurocalcin delta
• NCBI Official Symbol: NCALD
• NCBI Protein Information: neurocalcin-delta
• UniProt Protein Name: Neurocalcin-delta
• Protein Family: Neurocalcin
• UniProt Gene Name: NCALD
• UniProt Entry Name: NCALD_HUMAN

NCBI Description

This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

NCALD: May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions. Belongs to the recoverin family.

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: clathrin coat of trans-Golgi network vesicle; intracellular; cytosol

Molecular Function: tubulin binding; protein binding; clathrin binding; calcium ion binding; actin binding; alpha-tubulin binding

Biological Process: vesicle-mediated transport; synaptic transmission; regulation of systemic arterial blood pressure; calcium-mediated signaling