Goat RASIP1/RAIN Polyclonal Antibody | anti-RASIP1 antibody

Goat anti-RASIP1/RAIN Antibody

Cat. Num. AAA420231

• Gene Names: RASIP1; RAIN
• Reactivity: Expected from sequence similarity: Human
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: RASIP1/RAIN; Polyclonal Antibody; Goat anti-RASIP1/RAIN Antibody; RASIP1; Ras-interacting protein 1; RAIN; FLJ20401; Ras interacting protein 1; Ras-interacting protein 1 antibody; RAIN antibody; FLJ20401 antibody; RASIP1 antibody; Ras interacting protein 1 antibody; RASIP1 / RAIN; anti-RASIP1 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: CANYRHGPPVATSP
• Sequence Length: 963

• Applicable Applications for anti-RASIP1 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 8000.; Western Blot: Experiments gave bands at approx 95kDa, 75kDa and 45kDa in A549 Lung Carcinoma lysate after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the predicted size of 110kDa according to NP_060275. The detected bands were successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence CANYRHGPPVATSP, from the C Terminus of the protein sequence according to NP_060275.2.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 38570105
• NCBI GeneID: 54922
• NCBI Accession #: NP_060275.2
• NCBI GenBank Nucleotide #: NM_017805.2
• Molecular Weight: 103,457 Da
• NCBI Official Full Name: ras-interacting protein 1
• NCBI Official Synonym Full Names: Ras interacting protein 1
• NCBI Official Symbol: RASIP1
• NCBI Official Synonym Symbols: RAIN
• NCBI Protein Information: ras-interacting protein 1
• UniProt Protein Name: Ras-interacting protein 1
• Protein Family: Ras-interacting protein
• UniProt Gene Name: RASIP1
• UniProt Synonym Gene Names: Rain
• UniProt Entry Name: RAIN_HUMAN

Uniprot Description

RASIP1: Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells. May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS- mediated transformation. Negative regulator of amino acid starvation-induced autophagy.

Protein type: Cell cycle regulation; GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: Golgi stack; perinuclear region of cytoplasm

Biological Process: angiogenesis; branching morphogenesis of a tube; negative regulation of autophagy; regulation of GTPase activity; signal transduction; vasculogenesis