Rabbit anti-Human RASIP1 Polyclonal Antibody | anti-RASIP1 antibody

RASIP1, ID (Ras-interacting Protein 1, Rain)

Cat. Num. AAA624434

• Gene Names: RASIP1; RAIN; FLJ20401
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: RASIP1; Polyclonal Antibody; ID (Ras-interacting Protein 1; Rain); Anti -RASIP1; anti-RASIP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RASIP1.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-RASIP1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the Center region of human RASIP1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RASIP1 Antibody (Center) in K562 cell line lysates (35ug/lane). RASIP1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with RASIP1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using RASIP1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RASIP1 antibody

RASIP1 may act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation.

Product Categories/Family for anti-RASIP1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 38570105
• NCBI GeneID: 54922
• NCBI Accession #: NP_060275.2
• NCBI GenBank Nucleotide #: NM_017805.2
• UniProt Accession #: Q5U651; Q6U676; Q8IUR2; Q9NX72
• Molecular Weight: 103,457 Da
• NCBI Official Full Name: ras-interacting protein 1
• NCBI Official Synonym Full Names: Ras interacting protein 1
• NCBI Official Symbol: RASIP1
• NCBI Official Synonym Symbols: RAIN; FLJ20401
• NCBI Protein Information: ras-interacting protein 1
• UniProt Protein Name: Ras-interacting protein 1
• Protein Family: Ras-interacting protein
• UniProt Gene Name: RASIP1
• UniProt Entry Name: RAIN_HUMAN

Uniprot Description

RASIP1: Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells. May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS- mediated transformation. Negative regulator of amino acid starvation-induced autophagy.

Protein type: GAPs; Cell cycle regulation; GAPs, Ras

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: Golgi stack; perinuclear region of cytoplasm

Biological Process: negative regulation of autophagy; branching morphogenesis of a tube; angiogenesis; vasculogenesis; signal transduction