Rabbit RASGRP1 Polyclonal Antibody | anti-RASGRP1 antibody

RASGRP1 Antibody (Center)

Cat. Num. AAA9206589

• Gene Names: RASGRP1; V; RASGRP; hRasGRP1; CALDAG-GEFI; CALDAG-GEFII
• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RASGRP1; Polyclonal Antibody; RASGRP1 Antibody (Center); RAS guanyl-releasing protein 1; Calcium and DAG-regulated guanine nucleotide exchange factor II; CalDAG-GEFII; Ras guanyl-releasing protein; RASGRP; anti-RASGRP1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RASGRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-521 amino acids from the Central region of human RASGRP1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 495-521

• Applicable Applications for anti-RASGRP1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000; IHC-P~~1:10~50; FC~~1:10~50
• Function: Functions as a calcium- and diacylglycerol(DAG)- regulated nucleotide exchange factorspecifically activating Ras through theexchange of bound GDP for GTP (PubMed: 15899849,PubMed: 23908768). Activatesthe Erk/MAP kinase cascade (PubMed: 15899849). RegulatesT-cell/B-cell development, homeostasis anddifferentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras(PubMed: 10807788, PubMed: 12839994).Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways (PubMed: 19933860). Functions in mast cell degranulation and cytokinesecretion, regulating FcERI-evoked allergic responses (By similarity). May also function in differentiation of other cell types (PubMed: 12845332).
• Cellular Location: Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Golgiapparatus membrane; Peripheral membraneprotein. Endoplasmic reticulum membrane;Peripheral membrane protein. Note=Foundboth in the cytosol and associated with membranes. Relocalization to the cellmembrane upon activation is F-actin-dependent. Translocates to the Golgiin response to phorbol ester or nerve growth factor. Localizes to somata and dendrites but not to axons of hippocampal pyramidal cells (By similarity).
• Tissue Location: Expressed in brain with higher expression incerebellum, cerebral cortex and amygdala. Expressed in the hematopoietic system. Expressed in T-cells (at protein level)Expressed in NK cells (at protein level)(PubMed:19933860)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(RASGRP1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the RASGRP1 antibody detected the RASGRP1 protein (arrow).)

Immunohistochemistry (IHC)

(RASGRP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RASGRP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RASGRP1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-RASGRP1 antibody

Cancer; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 190684646
• NCBI GeneID: 10125
• NCBI Accession #: NP_001122074.1; NP_005730.2
• NCBI GenBank Nucleotide #: NM_001128602.1
• UniProt Accession #: O95267; Q56CZ0; Q58G75; Q59HB1; Q5I3A8; Q6GV31; Q6NX39; Q7LDG6; Q9UI94; Q9UNN9
• Molecular Weight: 90402
• NCBI Official Full Name: RAS guanyl-releasing protein 1 isoform b
• NCBI Official Synonym Full Names: RAS guanyl releasing protein 1 (calcium and DAG-regulated)
• NCBI Official Symbol: RASGRP1
• NCBI Official Synonym Symbols: V; RASGRP; hRasGRP1; CALDAG-GEFI; CALDAG-GEFII
• NCBI Protein Information: RAS guanyl-releasing protein 1
• UniProt Protein Name: RAS guanyl-releasing protein 1
• Protein Family: RAS guanyl-releasing protein
• UniProt Gene Name: RASGRP1
• UniProt Synonym Gene Names: RASGRP; CalDAG-GEFII
• UniProt Entry Name: GRP1_HUMAN

NCBI Description

This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE). [provided by RefSeq, Jul 2008]

Uniprot Description

RASGRP1: Functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Couples T-lymphocytes and B-lymphocytes antigen receptors to the activation of Ras. Hence, regulates T-cells and B-cells development, homeostasis and differentiation. Functions also in FcERI-evoked degranulation and cytokine secretion by mast cells, regulating allergic responses. May also function in other cell types differentiation. Defects in RASGRP1 may contribute to susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Aberrantly spliced isoforms and/or diminished levels of RASGRP1 are found in a cohort of SLE patients raising the possibility that dysregulation of this signaling protein contributes to the development of autoimmunity in a subset of SLE patients. Belongs to the RASGRP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Ras

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; mast cell granule; membrane; plasma membrane; cytosol

Molecular Function: guanyl-nucleotide exchange factor activity; calcium ion binding; lipid binding

Biological Process: platelet activation; inflammatory response to antigenic stimulus; Ras protein signal transduction; vesicle transport along microtubule; regulation of phosphoinositide 3-kinase cascade; innate immune response; cytokine production; mast cell degranulation; signal transduction; cell differentiation; blood coagulation; secretory granule localization