Rabbit RGS4 Polyclonal Antibody | anti-RGS4 antibody

RGS4 Polyclonal Antibody

Cat. Num. AAA3006476

• Gene Names: RGS4; RGP4; SCZD9
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RGS4; Polyclonal Antibody; RGS4 Polyclonal Antibody; Regulator of G-protein signaling 4; RGP4; RGS-4; anti-RGS4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RGS4 polyclonal antibody detects endogenous levels of RGS4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 205

• Applicable Applications for anti-RGS4 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human RGS4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RGS4 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:sp2/0 whole cell lysateLane3:PC12 whole cell lysate)

Related Product Information for anti-RGS4 antibody

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four Galpha GTPase-activating proteins (GAPs) have been identified and are designated RGS1 (regulator of G protein signaling), RGS4, RGS10 and GAIP (Galpha-interacting protein). Each of these proteins has been shown to deactivate specific Galpha isoforms by increasing the rate at which they convert GTP to GDP. RGS1, RGS4 and GAIP bind tightly to and exhibit GAP activity towards Galpha i, Galpha o and Galpha t, but not Galpha s. RGS10 increases the GTP hydrolytic activity of several members of the Galpha i subfamily including Galpha i-3, Galpha z, and Galpha o.

NCBI and Uniprot Product Information

• NCBI GI #: 5032039
• NCBI GeneID: 5999
• NCBI Accession #: NP_005604.1
• NCBI GenBank Nucleotide #: NP_005604.1
• UniProt Accession #: P49798
• Molecular Weight: ~ 23kDa
• NCBI Official Full Name: regulator of G-protein signaling 4 isoform 2
• NCBI Official Synonym Full Names: regulator of G protein signaling 4
• NCBI Official Symbol: RGS4
• NCBI Official Synonym Symbols: RGP4; SCZD9
• NCBI Protein Information: regulator of G-protein signaling 4
• UniProt Protein Name: Regulator of G-protein signaling 4
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS4
• UniProt Synonym Gene Names: RGP4; RGS4
• UniProt Entry Name: RGS4_HUMAN

NCBI Description

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS4: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein. Expressed in brain and heart. Expressed in brain at protein level. Expressed in prefontal and visual cortex. Isoform 4 and isoform 5 are expressed ubiquitously. Isoform 1, isoform 2 and isoform 3 are not expressed in the cerebellum. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: protein complex; cytoplasm; plasma membrane; cytosol

Molecular Function: calmodulin binding; G-protein alpha-subunit binding; GTPase activator activity

Biological Process: regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity; inactivation of MAPK activity

Disease: Schizophrenia 9