Rabbit anti-Mouse RASD1 Polyclonal Antibody | anti-RASD1 antibody

RASD1, CT (RASD1, AGS1, DEXRAS1, Dexamethasone-induced Ras-related protein 1, Activator of G-protein signaling 1) (Biotin)

Cat. Num. AAA6340429

• Gene Names: RASD1; AGS1; DEXRAS1; MGC:26290
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RASD1; Polyclonal Antibody; CT (RASD1; AGS1; DEXRAS1; Dexamethasone-induced Ras-related protein 1; Activator of G-protein signaling 1) (Biotin); Activator of G-protein signaling 1; anti-RASD1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse RASD1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RASD1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 248-277 in the C-terminal region of human RASD1, UniProt Accession #Q9Y272.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS6009131 in mouse liver tissue lysates (35ug/lane). This demonstrates the RASD1 antibody detected the RASD1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of MBS6009131 in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-RASD1 antibody

This gene encodes a Ras-related protein that is stimulated by dexamethasone. The exact function of this gene is unknown, but it may play a role in dexamethasone-induced alterations in cell morphology, growth and cell-extracellular matrix interactions. In addition, studies of a similar rat protein suggest that it functions as as a novel physiologic nitric oxide (NO) effector. The gene product belongs to the Ras superfamily of small GTPases.

Product Categories/Family for anti-RASD1 antibody

Antibodies; G Proteins

References

1. Nojima, M., et al. Clin. Cancer Res. 15(13):4356-4364(2009). 2. Lau, K.F., et al. J. Biol. Chem. 283(50):34728-34737(2008). 3. Attarzadeh-Yazdi, G., et al. Neurochem. Res. 33(4):609-613(2008). 4. Cheng, H.Y., et al. J. Neurosci. 26(50):12984-12995(2006). 5. Nguyen, C.H., et al. Mol. Pharmacol. 69(5):1763-1771(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 7706359
• NCBI GeneID: 51655
• NCBI Accession #: NP_057168.1
• NCBI GenBank Nucleotide #: NM_016084.4
• UniProt Accession #: Q9Y272; Q9NYB4; B2R709; Q9HC43
• Molecular Weight: 31,642 Da
• NCBI Official Full Name: dexamethasone-induced Ras-related protein 1 isoform 1 proprotein
• NCBI Official Synonym Full Names: RAS, dexamethasone-induced 1
• NCBI Official Symbol: RASD1
• NCBI Official Synonym Symbols: AGS1; DEXRAS1; MGC:26290
• NCBI Protein Information: dexamethasone-induced Ras-related protein 1; OTTHUMP00000065541; ras-related protein; activator of G-protein signaling 1
• UniProt Protein Name: Dexamethasone-induced Ras-related protein 1
• Protein Family: Dexamethasone-induced Ras-related protein
• UniProt Gene Name: RASD1
• UniProt Synonym Gene Names: AGS1; DEXRAS1
• UniProt Entry Name: RASD1_HUMAN

NCBI Description

This gene encodes a member of the Ras superfamily of small GTPases and is induced by dexamethasone. The protein is an activator of G-protein signaling and acts as a direct nucleotide exchange factor for Gi-Go proteins. This protein interacts with the neuronal nitric oxide adaptor protein CAPON, and a nuclear adaptor protein FE65 which interacts with the Alzheimer's disease amyloid precursor protein (APP). This gene may play a role in dexamethasone-induced alterations in cell morphology, growth and cell-extracellular matrix interactions. Epigenetic inactivation of this gene is closely correlated with resistance to dexamethasone in multiple myeloma cells. Alternatively spliced transcript variants encoding different isoforms have beeen found for this gene.

Uniprot Description

RASD1: Small GTPase. Negatively regulates the transcription regulation activity of the APBB1/FE65-APP complex via its interaction with APBB1/FE65. Forms a ternary complex with CAPON and NOS1. Component of a complex, at least composed of APBB1, RASD1/DEXRAS1 and APP. Interacts with APBB1/FE65. By dexamethasone. Expressed in a variety of tissues including heart, cardiovascular tissues, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, gastrointestinal and reproductive tissues. Belongs to the small GTPase superfamily. RasD family.

Protein type: G protein, monomeric; G protein, monomeric, RasD; G protein

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleus; perinuclear region of cytoplasm; plasma membrane; sarcoplasmic reticulum

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of transcription, DNA-dependent; nitric oxide mediated signal transduction; signal transduction; small GTPase mediated signal transduction