Rabbit anti-Human Dexras1 Polyclonal Antibody | anti-RASD1 antibody

Dexras1 (Ras dexamethasone induced 1)

Cat. Num. AAA620172

• Gene Names: RASD1; AGS1; DEXRAS1; MGC:26290
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Dexras1; Polyclonal Antibody; Dexras1 (Ras dexamethasone induced 1); Anti -Dexras1 (Ras dexamethasone induced 1); anti-RASD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Dexras1. Cross-reacts with a 60 kDa protein of unknown origin.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-RASD1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%; Tween-20 at 4 degree C with gentle shaking, overnight.
• Immunogen: Synthetic peptide corresponding to residues near the carboxy terminus of human Dexras1 protein (KLH-coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from HeLa and Hep G2 cells using MBS620172.)

Related Product Information for anti-RASD1 antibody

Dexras1 (Ras dexamethasone induced 1) belongs to the Ras superfamily of GTPases and was initially identified as a dexamethasone inducible gene (1,2). Dexras1 reportedly regulates several distinct signal transduction pathways, including MAPK signaling, NMDA receptor-nitric oxide-mediated signaling, and pathways involving adenylyl cyclases (3-5). Dexras1 can directly modulate FE65-APP-mediated transcription and regulate the photic sensitivity of the mammalian circadian clock (6,7).

Product Categories/Family for anti-RASD1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 7706359
• NCBI GeneID: 51655
• NCBI Accession #: NP_057168.1
• NCBI GenBank Nucleotide #: NM_016084.4
• UniProt Accession #: Q9Y272; Q9NYB4; B2R709; Q9HC43
• Molecular Weight: 31,642 Da
• NCBI Official Full Name: dexamethasone-induced Ras-related protein 1 isoform 1 proprotein
• NCBI Official Synonym Full Names: RAS, dexamethasone-induced 1
• NCBI Official Symbol: RASD1
• NCBI Official Synonym Symbols: AGS1; DEXRAS1; MGC:26290
• NCBI Protein Information: dexamethasone-induced Ras-related protein 1; OTTHUMP00000065541; ras-related protein; activator of G-protein signaling 1
• UniProt Protein Name: Dexamethasone-induced Ras-related protein 1
• Protein Family: Dexamethasone-induced Ras-related protein
• UniProt Gene Name: RASD1
• UniProt Synonym Gene Names: AGS1; DEXRAS1
• UniProt Entry Name: RASD1_HUMAN

NCBI Description

This gene encodes a member of the Ras superfamily of small GTPases and is induced by dexamethasone. The protein is an activator of G-protein signaling and acts as a direct nucleotide exchange factor for Gi-Go proteins. This protein interacts with the neuronal nitric oxide adaptor protein CAPON, and a nuclear adaptor protein FE65 which interacts with the Alzheimer's disease amyloid precursor protein (APP). This gene may play a role in dexamethasone-induced alterations in cell morphology, growth and cell-extracellular matrix interactions. Epigenetic inactivation of this gene is closely correlated with resistance to dexamethasone in multiple myeloma cells. Alternatively spliced transcript variants encoding different isoforms have beeen found for this gene.

Uniprot Description

RASD1: Small GTPase. Negatively regulates the transcription regulation activity of the APBB1/FE65-APP complex via its interaction with APBB1/FE65. Forms a ternary complex with CAPON and NOS1. Component of a complex, at least composed of APBB1, RASD1/DEXRAS1 and APP. Interacts with APBB1/FE65. By dexamethasone. Expressed in a variety of tissues including heart, cardiovascular tissues, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, gastrointestinal and reproductive tissues. Belongs to the small GTPase superfamily. RasD family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, RasD

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: perinuclear region of cytoplasm; plasma membrane; nucleus

Molecular Function: GTPase activity; GTP binding

Biological Process: G-protein coupled receptor protein signaling pathway; metabolic process; small GTPase mediated signal transduction; negative regulation of transcription, DNA-dependent; signal transduction; nitric oxide mediated signal transduction