Rabbit anti-Human Ras-GRF1 Polyclonal Antibody | anti-Ras-GRF1 antibody

Ras-GRF1 (Phospho-Ser916) Antibody

Cat. Num. AAA9402198

• Gene Names: Rasgrf1; Gnrp; Grf1; p190; CDC25; P190-A; CDC25Mm; Grfbeta; AI844718; Ras-GRF1; p190RhoGEF
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: Ras-GRF1; Polyclonal Antibody; Ras-GRF1 (Phospho-Ser916) Antibody; CDC25; GNRP; GRF1; RASGRF1; anti-Ras-GRF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1262

• Applicable Applications for anti-Ras-GRF1 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Serine 916(R-M-S(p)-L-A) derived from Mouse Ras-GRF1.
• Modification: Phospho-Ser916
• Target Name: Ras-GRF1
• Preparation and Storage: Store at -20 degree C/1 year

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Related Product Information for anti-Ras-GRF1 antibody

Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx. Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

Product Categories/Family for anti-Ras-GRF1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 6755288
• NCBI GeneID: 19417
• NCBI Accession #: NP_035375.1
• NCBI GenBank Nucleotide #: NM_011245.2
• UniProt Accession #: P27671
• Molecular Weight: 144,102 Da
• NCBI Official Full Name: ras-specific guanine nucleotide-releasing factor 1 isoform 1
• NCBI Official Synonym Full Names: RAS protein-specific guanine nucleotide-releasing factor 1
• NCBI Official Symbol: Rasgrf1
• NCBI Official Synonym Symbols: Gnrp; Grf1; p190; CDC25; P190-A; CDC25Mm; Grfbeta; AI844718; Ras-GRF1; p190RhoGEF
• NCBI Protein Information: ras-specific guanine nucleotide-releasing factor 1
• UniProt Protein Name: Ras-specific guanine nucleotide-releasing factor 1
• UniProt Gene Name: Rasgrf1
• UniProt Synonym Gene Names: Cdc25; Grf1; Ras-GRF1; GNRP
• UniProt Entry Name: RGRF1_MOUSE

Uniprot Description

RASGRF1: a guanine nucleotide exchange factor (GEF). Stimulates the dissociation of GDP from RAS protein. Activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit in the mouse brain,. The signaling pathway mediated by this protein may be important for long-term memory. Two alternatively spliced variants have been reported.

Protein type: GEFs; GEFs, Ras

Cellular Component: growth cone; intracellular; cytosol

Molecular Function: protein C-terminus binding; protein binding; Rho guanyl-nucleotide exchange factor activity; glutamate receptor binding; guanyl-nucleotide exchange factor activity; protein kinase binding

Biological Process: regulation of Ras protein signal transduction; regulation of Rac protein signal transduction; cell proliferation; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; regulation of Rho protein signal transduction; regulation of synaptic plasticity; positive regulation of Ras protein signal transduction; neurite development; regulation of neuronal synaptic plasticity; positive regulation of GTPase activity