Rabbit PEA15 Polyclonal Antibody | anti-PEA15 antibody

Phospho-PEA15 (Ser116) Antibody

Cat. Num. AAA9613274

• Gene Names: PEA15; PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: PEA15; Polyclonal Antibody; Phospho-PEA15 (Ser116) Antibody; 15kDa phosphoprotein enriched in astrocytes; Astrocytic phosphoprotein PEA 15; Astrocytic phosphoprotein PEA-15; Astrocytic phosphoprotein PEA15; HMAT 1; HMAT1; Homolog of mouse MAT 1 oncogene; Homolog of mouse MAT1 oncogene; HUMMAT 1H; HUMMAT1H; MAT 1; MAT 1H; MAT1; MAT1H; PEA 15; Pea15; PEA15 protein; PEA15_HUMAN; PED; Phosphoprotein enriched in astrocytes 15; Phosphoprotein enriched in astrocytes 15kD; Phosphoprotein enriched in diabetes; anti-PEA15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-PEA15 (Ser116) Antibody detects endogenous levels of PEA15 only when phosphorylated at Ser116.; Tissue Specificity: Ubiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PEA15 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human PEA15 around the phosphorylation site of Ser116.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.
• Subunit Structure: Binds RPS6KA3, MAPK3 and MAPK1. Transient interaction with PLD1 and PLD2 (By similarity). Interacts with CASP8 and FADD.
• Subcellular Location: Cytoplasm.; Note: Associated with microtubules.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of PEA-15 (Phospho-Ser116) in lysates of A172 200 nM TPA, using PEA-15 (Phospho-Ser116) Antibody(AF4474).)

Related Product Information for anti-PEA15 antibody

Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.

NCBI and Uniprot Product Information

• NCBI GI #: 4505705
• NCBI GeneID: 8682
• NCBI Accession #: NP_003759.1
• NCBI GenBank Nucleotide #: NM_003768.3
• UniProt Accession #: Q15121; O00511
• Molecular Weight: 15,040 Da
• NCBI Official Full Name: astrocytic phosphoprotein PEA-15
• NCBI Official Synonym Full Names: phosphoprotein enriched in astrocytes 15
• NCBI Official Symbol: PEA15
• NCBI Official Synonym Symbols: PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H
• NCBI Protein Information: astrocytic phosphoprotein PEA-15; homolog of mouse MAT-1 oncogene; phosphoprotein enriched in diabetes; Phosphoprotein enriched in astrocytes, 15kD; 15 kDa phosphoprotein enriched in astrocytes
• UniProt Protein Name: Astrocytic phosphoprotein PEA-15
• Protein Family: Astrocytic phosphoprotein
• UniProt Gene Name: PEA15
• UniProt Synonym Gene Names: PED
• UniProt Entry Name: PEA15_HUMAN

NCBI Description

This gene encodes a death effector domain-containing protein, which is a major phosphoprotein in astrocytes, and an endogenous substrate for protein kinase C. Studies using knockout mice suggest that this protein may protect astrocytes from TNF-induced apoptosis. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. [provided by RefSeq, Sep 2011]

Uniprot Description

PEA-15: an ubiquitously expressed anti-apoptotic protein. Inhibits both Fas- and Tnfrsf1a/Tnfr1-mediated caspase-8 activity and apoptosis. Interacts with CASP8/FLICE and FADD. Regulates glucose transport by controlling both the content of GLUT1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of GLUT4 from the cell interior to the surface. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.

Protein type: Motility/polarity/chemotaxis; Apoptosis

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: microtubule associated complex; cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of glucose import; apoptosis; transport; DNA damage checkpoint; carbohydrate transport; response to morphine