Rabbit 14-3-3 zeta/ delta Polyclonal Antibody | anti-14-3-3 zeta/ delta antibody

14-3-3 zeta/ delta (Phospho-Thr232) Antibody

Cat. Num. AAA9410569

• Gene Names: YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: 14-3-3 zeta/ delta; Polyclonal Antibody; 14-3-3 zeta/ delta (Phospho-Thr232) Antibody; 1433Z; 143Z; FAS; YWHAZ; anti-14-3-3 zeta/ delta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of 14-3-3 zeta/ delta only when phosphorylated at threonine 232.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 245

• Applicable Applications for anti-14-3-3 zeta/ delta antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of threonine232(S-D-T(p)-Q-G) derived from Human 14-3-3 zeta/ delta.
• Modification: Phospho-Thr232
• Target Name: 14-3-3 zeta/ delta
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells treated with UV using Phospho-14-3-3 zeta/ delta (Thr232) antibody.The lane on the right is treated with the antigen-specific peptide.)

Related Product Information for anti-14-3-3 zeta/ delta antibody

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.Sluchanko NN, et al. (2011) Arch Biochem Biophys 506, 24-34 Sluchanko NN, et al. (2008) Arch Biochem Biophys 477, 305-12 Clokie SJ, et al. (2005) FEBS J 272, 3767-76

Product Categories/Family for anti-14-3-3 zeta/ delta antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 208973238
• NCBI GeneID: 7534
• NCBI Accession #: NP_001129171.1
• NCBI GenBank Nucleotide #: NM_001135699.1
• UniProt Accession #: P63104; P29213; P29312; Q32P43; Q5XJ08; Q6GPI2; Q6IN74; Q6NUR9; Q6P3U9; Q86V33; A8K1N0; B7Z465
• Molecular Weight: 19,333 Da
• NCBI Official Full Name: 14-3-3 protein zeta/delta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
• NCBI Official Symbol: YWHAZ
• NCBI Official Synonym Symbols: HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta
• NCBI Protein Information: 14-3-3 protein zeta/delta
• UniProt Protein Name: 14-3-3 protein zeta/delta
• UniProt Gene Name: YWHAZ
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433Z_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; protein complex; mast cell granule; cytoplasmic vesicle membrane; focal adhesion; mitochondrion; postsynaptic density; leading edge; cytosol; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; melanosome; nucleus

Molecular Function: protein domain specific binding; identical protein binding; protein binding; protein complex binding; protein kinase binding; transcription factor binding

Biological Process: response to drug; platelet activation; histamine secretion by mast cell; establishment of Golgi localization; apoptosis; gene expression; blood coagulation; signal transduction; protein targeting to mitochondrion; negative regulation of apoptosis