Factor XIIIa Assay Kit | F13A1 assay kit

Factor XIIIa Activity Assay Kit (Colorimetric)

Cat. Num. AAA846806

• Gene Names: F13A1; F13A
• Synonyms: Factor XIIIa; Factor XIIIa Activity Assay Kit (Colorimetric); F13A1 assay kit

• Sequence Length: 732

• Application Notes: Measurement of Factor XIIIa activity in plasma samples and purified/crude enzyme preparations
• Preparation and Storage: Store at -20 degree C for 12 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F13A1 assay kit

Background/Introduction: Factor XIII or fibrin stabilizing factor (EC 2.3.2.13) is an enzyme involved in the blood coagulation system that crosslinks fibrin. Factor XIII is a transglutaminase that circulates in the plasma as a heterotetramer of two catalytic "A" subunits and two carrier "B" subunits. Factor XIII is activated by thrombin in the presence of calcium which leads to the release of the activation peptide, followed by the dissociation of carrier subunits. The activated Factor XIII (XIIIa) acts on fibrin (Factor Ia) to form gamma-glutamyl-?-lysyl amide cross links between fibrin molecules leading to the formation of an insoluble clot. The deficiency of Factor XIII affects fibrin clot stability and bleeding disorders. Factor XIIIa activity assay kit utilizes the transglutaminase activity of factor XIIIa to cross link an amine-containing substrate to glutamine-containing substrate resulting in the loss of ammonia which can be quantitatively measured by a colorimetric assay. The kit is easy-to-use and can detect Factor XIIIa (as low as 0.1 Loewy U) from plasma and purified protein samples.

Product Categories/Family for F13A1 assay kit

Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 412153
• NCBI GeneID: 2162
• Molecular Weight: 83,267 Da
• NCBI Official Full Name: factor XIIIa
• NCBI Official Synonym Full Names: coagulation factor XIII A chain
• NCBI Official Symbol: F13A1
• NCBI Official Synonym Symbols: F13A
• NCBI Protein Information: coagulation factor XIII A chain
• UniProt Protein Name: Coagulation factor XIII A chain
• Protein Family: Coagulation factor
• UniProt Gene Name: F13A1
• UniProt Synonym Gene Names: F13A; Coagulation factor XIIIa

NCBI Description

This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]

Uniprot Description

F13A1: Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD). FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. Belongs to the transglutaminase superfamily. Transglutaminase family.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 6p25.1

Cellular Component: extracellular region

Molecular Function: protein-glutamine gamma-glutamyltransferase activity

Biological Process: blood coagulation; peptide cross-linking; platelet degranulation

Disease: Factor Xiii, A Subunit, Deficiency Of; Myocardial Infarction, Susceptibility To; Thrombophilia Due To Thrombin Defect