Rabbit anti-Human CSNK2B Polyclonal Antibody | anti-CSNK2B antibody

CSNK2B, CT (F168) (Casein Kinase II beta Subunit, CK II beta, Phosvitin, G5a, CK2N, G5A) (APC)

Cat. Num. AAA6467417

• Gene Names: CSNK2B; G5A; CK2B; CK2N; Ckb1; Ckb2; CSK2B
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CSNK2B; Polyclonal Antibody; CT (F168) (Casein Kinase II beta Subunit; CK II beta; Phosvitin; G5a; CK2N; G5A) (APC); anti-CSNK2B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CSNK2B.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CSNK2B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CSNK2B (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CSNK2B (arrow) using rabbit polyclonal CSNK2B Antibody (C-term F168) (RB09717). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CSNK2B gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-CSNK2B antibody

Antibodies; Protein Kinases

References

1. Schwartz, E.I., et al., Mol. Cell. Biol. 24(21):9580-9591 (2004). 2. Lee, G., et al., J. Biol. Chem. 279(8):6834-6839 (2004). 3. Lim, A.C., et al., J. Biol. Chem. 279(6):4433-4439 (2004). 4. Singh, D.K., et al., Virology 313(2):435-451 (2003). 5. Szebeni, A., et al., J. Biol. Chem. 278(11):9107-9115 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 23503295
• NCBI GeneID: 1460
• NCBI Accession #: NP_001311
• NCBI GenBank Nucleotide #: NM_001320.6
• UniProt Accession #: P67870; P07312; P13862; Q4VX47; B0UXA9
• Molecular Weight: 24.9kDa (215aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: casein kinase II subunit beta isoform 1
• NCBI Official Synonym Full Names: casein kinase 2 beta
• NCBI Official Symbol: CSNK2B
• NCBI Official Synonym Symbols: G5A; CK2B; CK2N; Ckb1; Ckb2; CSK2B
• NCBI Protein Information: casein kinase II subunit beta
• UniProt Protein Name: Casein kinase II subunit beta
• UniProt Gene Name: CSNK2B
• UniProt Synonym Gene Names: CK2N; G5A; CK II beta
• UniProt Entry Name: CSK2B_HUMAN

NCBI Description

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

CK2B: a regulatory subunit of the casein kinase 2 holoenzyme. Exists as a tetramer composed of two catalytic subunits, alpha and alpha-prime, and two regulatory beta subunits. The beta subunits undergo autophosphorylation. Plays a complex role in regulating the basal catalytic activity of the alpha subunit. Participates in Wnt signaling

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytoplasm; plasma membrane; protein kinase CK2 complex; PcG protein complex; nucleus; cytosol

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein domain specific binding; protein binding; metal ion binding; protein kinase regulator activity; transcription factor binding; receptor binding

Biological Process: negative regulation of cell proliferation; axon guidance; positive regulation of activin receptor signaling pathway; Wnt receptor signaling pathway; cellular protein complex assembly; regulation of DNA binding; adiponectin-mediated signaling pathway; mitotic cell cycle; regulation of protein kinase activity; negative regulation of blood vessel endothelial cell migration; signal transduction; protein amino acid phosphorylation