Rabbit RAPGEF5 Polyclonal Antibody | anti-RAPGEF5 antibody

RAPGEF5 Polyclonal Antibody

Cat. Num. AAA3006146

• Gene Names: RAPGEF5; GFR; REPAC; MR-GEF
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RAPGEF5; Polyclonal Antibody; RAPGEF5 Polyclonal Antibody; Rap guanine nucleotide exchange factor 5; Guanine nucleotide exchange factor for Rap1; M-Ras-regulated Rap GEF; MR-GEF; Related to Epac; Repac; GFR; KIAA0277; MRGEF; anti-RAPGEF5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RAPGEF5 polyclonal antibody detects endogenous levels of RAPGEF5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 730

• Applicable Applications for anti-RAPGEF5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 476-521 of Human RAPGEF5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RAPGEF5 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Raw264. 7 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-RAPGEF5 antibody

Members of the RAS subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as Repac, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation. Repac, also known as Rap guanine nucleotide exchange factor 5 (RAPGEF5), is a 580 amino acid protein that is expressed in brain and testis, with weaker expression in heart, placenta, lung, pancreas and small intestine. Existing as two alternatively spliced isoforms, the Repac gene is conserved in cow, mouse and rat. The gene that encodes Repac maps to human chromosome 7p15.3, with high expression of this region linking to positive response to erlotinib therapy for those with non-small-cell lung cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 119392077
• NCBI GeneID: 9771
• NCBI Accession #: NP_036426.3
• NCBI GenBank Nucleotide #: NP_036426.3
• UniProt Accession #: Q92565
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: rap guanine nucleotide exchange factor 5
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor 5
• NCBI Official Symbol: RAPGEF5
• NCBI Official Synonym Symbols: GFR; REPAC; MR-GEF
• NCBI Protein Information: rap guanine nucleotide exchange factor 5
• UniProt Protein Name: Rap guanine nucleotide exchange factor 5
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEF5
• UniProt Synonym Gene Names: GFR; KIAA0277; MRGEF; MR-GEF; Repac
• UniProt Entry Name: RPGF5_HUMAN

NCBI Description

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]

Uniprot Description

RAPGEF5: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: nucleus

Molecular Function: Rap guanyl-nucleotide exchange factor activity; GTP-dependent protein binding; Ras guanyl-nucleotide exchange factor activity

Biological Process: nervous system development; small GTPase mediated signal transduction