Rabbit eIF4E Polyclonal Antibody | anti-eIF4E antibody

Anti-eIF4E Antibody, Rabbit Polyclonal

Cat. Num. AAA8100985

• Gene Names: EIF4E; CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: eIF4E; Polyclonal Antibody; Anti-eIF4E Antibody; Rabbit Polyclonal; eIF4E Antibody; Rabbit PAb; Antigen Affinity Purified; eukaryotic translation initiation factor 4E; Anti-AUTS19 Antibody; Anti-CBP Antibody; Anti-EIF4E1 Antibody; Anti-EIF4EL1 Antibody; Anti-EIF4F Antibody; anti-eIF4E antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human eIF4E
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-eIF4E antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human eIF4E
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human eIF4E, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human EIF4E in human hepatoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human EIF4E in human breast carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-eIF4E antibody

The eukaryotic translation initiation factor 4E (eIF4E), is a limiting component of the eIF4F complex which is a critical determinant for the translation of mRNAs. We had shown that eIF4E availability and phosphorylation promote metastasis in mouse models of breast cancer by selectively augmenting the translation of mRNAs involved in invasion and metastasis. eIF4E phosphorylation is required for the chronic action of antidepressants, such as fluoxetine in mice. Elevated levels of eukaryotic initiation factor 4E (eIF4E) are implicated in neoplasia, with cumulative evidence pointing to its role in the etiopathogenesis of hematological diseases. eIF4E is a key focus in cancer research due to its role in controlling the translation of tumour-associated proteins, that drive an aggressive migratory phenotype.

NCBI and Uniprot Product Information

• NCBI GI #: 194578907
• NCBI GeneID: 1977
• NCBI Accession #: NP_001124150.1
• NCBI GenBank Nucleotide #: NM_001130678.1
• UniProt Accession #: P06730; Q96E95; B7Z6V1; D6RCQ6
• Molecular Weight: 25,097 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 4E isoform 3
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E
• NCBI Official Symbol: EIF4E
• NCBI Official Synonym Symbols: CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
• NCBI Protein Information: eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; eukaryotic translation initiation factor 4E-like 1
• UniProt Protein Name: Eukaryotic translation initiation factor 4E
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF4E
• UniProt Synonym Gene Names: EIF4EL1; EIF4F; eIF-4E; eIF4E
• UniProt Entry Name: IF4E_HUMAN

NCBI Description

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: mRNA cap complex; stress granule; cytoplasm; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding

Biological Process: translation; viral reproduction; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; lung development; G1/S transition of mitotic cell cycle

Disease: Autism, Susceptibility To, 19