Western Blot (WB)
(HSPD1 Antibody (Center) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the HSPD1 antibody detected the HSPD1 protein (arrow).)
Rabbit anti-Human HSPD1 Polyclonal Antibody | anti-HSPD1 antibody
HSPD1, ID (60kD Heat Shock Protein, Mitochondrial, Heat Shock Protein 60, 60kD Chaperonin, Chaperonin 60, Mitochondrial Matrix Protein P1, P60 Lymphocyte Protein, HuCHA60, HSP60) (AP)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(HSPD1 Antibody (Center) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the HSPD1 antibody detected the HSPD1 protein (arrow).)
Western Blot (WB)
(HSPD1 Antibody (Center) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the HSPD1 antibody detected the HSPD1 protein (arrow).)
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with HSPD1 (Center) antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2859C HSPD1 (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Note the highly specific localization of the HSPD1 mainly to the mitochondria, supported by Human Protein Atlas Data.)
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with HSPD1 (Center) antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2859C HSPD1 (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Note the highly specific localization of the HSPD1 mainly to the mitochondria, supported by Human Protein Atlas Data.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HSPD1 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HSPD1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HSPD1 immunoreactivity is localized to Mitochondria significantly. )
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HSPD1 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HSPD1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HSPD1 immunoreactivity is localized to Mitochondria significantly. )
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
Uniprot Description
HSP60: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Interacts with HRAS. Interacts with HBV protein X and HTLV-1 protein p40tax. Belongs to the chaperonin (HSP60) family.
Protein type: Chaperone; Mitochondrial
Chromosomal Location of Human Ortholog: 2q33.1
Cellular Component: extracellular space; protein complex; cell surface; mitochondrion; early endosome; coated pit; cytosol; secretory granule; membrane; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; plasma membrane; lipopolysaccharide receptor complex; cyclin-dependent protein kinase activating kinase holoenzyme complex
Molecular Function: protein binding; lipopolysaccharide binding; p53 binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; double-stranded RNA binding; ATPase activity; DNA replication origin binding; ATP binding; single-stranded DNA binding
Biological Process: caspase activation; B cell proliferation; viral reproduction; B cell activation; protein stabilization; T cell activation; positive regulation of interleukin-12 production; positive regulation of apoptosis; protein maturation; isotype switching to IgG isotypes; positive regulation of interleukin-6 production; B cell cytokine production; positive regulation of interleukin-10 production; response to unfolded protein; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; 'de novo' protein folding; protein refolding; positive regulation of T cell mediated immune response to tumor cell; positive regulation of T cell activation; chaperone-mediated protein complex assembly; positive regulation of interferon-alpha production; negative regulation of apoptosis; positive regulation of macrophage activation
Disease: Spastic Paraplegia 13, Autosomal Dominant; Leukodystrophy, Hypomyelinating, 4
Research Articles on HSPD1
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Product Notes
The HSPD1 hspd1 (Catalog #AAA6479751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPD1, ID (60kD Heat Shock Protein, Mitochondrial, Heat Shock Protein 60, 60kD Chaperonin, Chaperonin 60, Mitochondrial Matrix Protein P1, P60 Lymphocyte Protein, HuCHA60, HSP60) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSPD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HSPD1 hspd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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