Rabbit anti-Human PIAS1 Polyclonal Antibody | anti-PIAS1 antibody

PIAS1, CT E3 SUMO-protein Ligase PIAS1, Protein Inhibitor of Activated STAT Protein 1, Gu Binding Protein, GBP, RNA Helicase II Binding Protein, DEAD/H Box-binding Protein 1, DDXBP1) (FITC)

Cat. Num. AAA6493369

• Gene Names: PIAS1; GBP; ZMIZ3; DDXBP1; GU/RH-II
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: PIAS1; Polyclonal Antibody; CT E3 SUMO-protein Ligase PIAS1; Protein Inhibitor of Activated STAT Protein 1; Gu Binding Protein; GBP; RNA Helicase II Binding Protein; DEAD/H Box-binding Protein 1; DDXBP1) (FITC); anti-PIAS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIAS1.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PIAS1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 100-130 in the N-terminal region of human PIAS1, UniProt Accession #O75925.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MBS628323 to detect PIAS1 in HL-60 tissue lysate.)

Western Blot (WB)

(Western blot analysis of PIAS1 (arrow) using MBS628323 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS628323 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PIAS1 antibody

Antibodies; Transcription Factors; STAT

References

1. Miyauchi, Y., et al., J. Biol. Chem. 277(51):50131-50136 (2002). 2. Nishida, T., et al., J. Biol. Chem. 277(44):41311-41317 (2002). 3. Tan, J.A., et al., J. Biol. Chem. 277(19):16993-17001 (2002). 4. Megidish, T., et al., J. Biol. Chem. 277(10):8255-8259 (2002). 5. Liu, B., et al., J. Biol. Chem. 276(39):36624-36631 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 7706637
• NCBI GeneID: 8554
• NCBI Accession #: NP_057250
• NCBI GenBank Nucleotide #: NM_016166.1
• UniProt Accession #: O75925; Q147X4; Q99751; Q9UN02; B2RB67; B3KSY9; C5J4B4
• Molecular Weight: 36,204 Da
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS1
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT, 1
• NCBI Official Symbol: PIAS1
• NCBI Official Synonym Symbols: GBP; ZMIZ3; DDXBP1; GU/RH-II
• NCBI Protein Information: E3 SUMO-protein ligase PIAS1; gu-binding protein; AR interacting protein; DEAD/H box-binding protein 1; RNA helicase II-binding protein; zinc finger, MIZ-type containing 3; protein inhibitor of activated STAT-1; protein inhibitor of activated STAT protein
• UniProt Protein Name: E3 SUMO-protein ligase PIAS1
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: PIAS1
• UniProt Synonym Gene Names: DDXBP1; GBP
• UniProt Entry Name: PIAS1_HUMAN

NCBI Description

This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

PIAS1: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Interacts with NCOA2 and AR. Interacts with NR2C1; the interaction promotes its sumoylation. Interacts with DDX21, CSRP2, AXIN1, JUN, UBE2I, SUMO1, SATB2, PLAG1, TP53 and STAT1 (dimer), following IFNA1-stimulation. Interacts with SP3 (preferentially when SUMO-modified). Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with STAT1. Interacts with CHUK/IKKA; this interaction induces PIAS1 phosphorylation. Interacts with PTK2/FAK1; the interaction promotes its sumoylation. Interacts with DDX5. Expressed in numerous tissues with highest level in testis. Belongs to the PIAS family.

Protein type: SUMO conjugating system; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 15q

Cellular Component: nucleoplasm; PML body; nuclear speck; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; DNA binding; androgen receptor binding; zinc ion binding; ubiquitin protein ligase binding; transcription coactivator activity; transcription corepressor activity; ligase activity

Biological Process: positive regulation of protein sumoylation; protein sumoylation; transcription, DNA-dependent; positive regulation of smooth muscle cell differentiation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; JAK-STAT cascade; protein-DNA complex assembly; regulation of cell proliferation