Western Blot (WB)
(Anti-RALY rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa)
Rabbit anti-Human RALY Polyclonal Antibody | anti-RALY antibody
Anti-RALY Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
RALY; Polyclonal Antibody; Anti-RALY Antibody; Rabbit Polyclonal; RALY Antibody; Rabbit PAb; Antigen Affinity Purified; RALY heterogeneous nuclear ribonucleoprotein; Anti-HNRPCL2 Antibody; Anti-P542 Antibody; anti-RALY antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human RALY
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-RALY antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ICC/IF: 1:100-1:500
IP: 5-10uL/mg of lysate
ICC/IF: 1:100-1:500
IP: 5-10uL/mg of lysate
Immunogen
E Coli-derived Human RALY fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human RALY fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-RALY rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa)
Western Blot (WB)
(Anti-RALY rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa)
Immunofluorescence (IF)
(Immunofluorescence staining of RALY in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RALY polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of RALY in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RALY polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
Immunoprecipitation (IP)
(RALY was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-RALY rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RALY rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa Observed band size :40 kDa)
Immunoprecipitation (IP)
(RALY was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-RALY rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RALY rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa Observed band size :40 kDa)
Related Product Information for anti-RALY antibody
RALY is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP), an RNA-binding protein that plays a role in mRNA splicing and metabolism, may be involved in tumorigenesis and development. RALY is a poly-U binding protein and a regulator of H1FX and ANXA1 in mammalian cells. The hnRNP family binds poly-U-rich elements within several RNAs and regulates the expression of specific transcripts. RALY is up-regulated in different types of cancer, and its down-regulation impairs cell cycle progression. Some studies have shown that RALY plays a role in promoting cancer in a variety of tumors. Targeting RALY could be a promising molecular target for non-small cell lung cancer (NSCLC) treatment. RALY plays a critical role in promoting invasiveness and is associated with poor prognosis in different types of cancers.
References
Tenzer S, et al. (2013) Proteome-wide characterization of the rna-binding protein raly-interactome using the in vivo-biotinylation-pulldown-quant (ibiopq) approach. J Proteome Res 12 (6): 2869-2884.Song G, et al. (2020) Raly may cause an aggressive biological behavior and a dismal prognosis in non-small-cell lung cancer. Exp Cell Res 389 (2): 111884.Rossi A, et al. (2017) Identification and dynamic changes of rnas isolated from raly-containing ribonucleoprotein complexes. Nucleic Acids Res 45 (11): 6775-6792.Cornella N, et al. (2017) The hnrnp raly regulates transcription and cell proliferation by modulating the expression of specific factors including the proliferation marker e2f1. J Biol Chem 292 (48): 19674-19692.
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Product Notes
The RALY (Catalog #AAA8108833) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RALY Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RALY can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ICC/IF: 1:100-1:500 IP: 5-10uL/mg of lysate. Researchers should empirically determine the suitability of the RALY for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RALY, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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