Rabbit RACK1 Polyclonal Antibody | anti-RACK1 antibody

RACK1 Polyclonal Antibody

Cat. Num. AAA2554983

• Gene Names: RACK1; H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: RACK1; Polyclonal Antibody; RACK1 Polyclonal Antibody; Cell proliferation-inducing gene 21 protein; GBLP; Gnb2-rs1; Gnb2l1; Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1; Guanine nucleotide binding protein beta polypeptide 2 like 1; Guanine nucleotide binding protein beta subunit 2 like 1; Guanine nucleotide binding protein beta subunit like protein 12.3; Guanine nucleotide binding protein subunit beta 2 like 1; Guanine nucleotide binding protein subunit beta like protein 12.3; Guanine nucleotide-binding protein subunit beta-2-like 1; Guanine nucleotide-binding protein subunit beta-like protein 12.3; H12.3; HLC-7; Human lung cancer oncogene 7 protein; lung cancer oncogene 7; OTTHUMP00000223704; OTTHUMP00000223870; OTTHUMP00000223891; OTTHUMP00000223893; OTTHUMP00000223900; OTTHUMP00000223902; OTTHUMP00000223930; OTTHUMP00000223931; PIG21; Proliferation inducing gene 21; Protein homologous to chicken B complex protein guanine nucleotide binding; Receptor for activated C kinase 1; Receptor for activated C kinase; Receptor of activated protein kinase C 1; anti-RACK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 317

• Applicable Applications for anti-RACK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant protein of human GNB2L1
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using GNB2L1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using GNB2L1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon using GNB2L1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cells using GNB2L1 antibody.)

Product Categories/Family for anti-RACK1 antibody

Cancer; Cell Biology; Signal transduction

NCBI and Uniprot Product Information

• NCBI GI #: 5174447
• NCBI GeneID: 10399
• NCBI Accession #: NP_006089.1
• NCBI GenBank Nucleotide #: NM_006098.4
• UniProt Accession #: P63244; P25388; P99049; Q53HU2; Q5J8M6; Q5VLR4; Q6FH47; B3KTJ0; D3DWS0
• Molecular Weight: Calculated: 35kDa; Observed: 35kDa
• NCBI Official Full Name: receptor of activated protein C kinase 1
• NCBI Official Synonym Full Names: receptor for activated C kinase 1
• NCBI Official Symbol: RACK1
• NCBI Official Synonym Symbols: H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
• NCBI Protein Information: receptor of activated protein C kinase 1
• UniProt Protein Name: Receptor of activated protein C kinase 1
• Protein Family: Receptor of activated protein C kinase
• UniProt Gene Name: RACK1
• UniProt Synonym Gene Names: HLC-7

Uniprot Description

Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression (PubMed:23636399). Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits (PubMed:28132843). Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (PubMed:19674157). Promotes migration of breast carcinoma cells by binding to and activating RHOA (PubMed:20499158).