PYCR1 active protein

PYCR1, 1-319aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203950

• Gene Names: PYCR1; P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PYCR1; 1-319aa; Human; His tag; E Coli (Bioactivity Validated); Pyrroline-5-carboxylate reductase 1; ARCL2B; P5C; P5CR; PIG45; PP222; PRO3; PYCR1 active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.5) containing 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDLA TVSALRKMGV KLTPHNKETV QHSDVLFLAV KPHIIPFILD EIGADIEDRH IVVSCAAGVT ISSIEKKLSA FRPAPRVIRC MTNTPVVVRE GATVYATGTH AQVEDGRLME QLLSSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR LAVRLGAQAL LGAAKMLLHS EQHPGQLKDN VSSPGGATIH ALHVLESGGF RSLLINAVEA SCIRTRELQS MADQEQVSPA AIKKTILDKV KLDSPAGTAL SPSGHTKLLP RSLAPAGKD
• Sequence Length: 319

• Applicable Applications for PYCR1 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 0.03 unit/mg. One unit will oxidize 1.0 umole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25C.
• Assay: 1. Prepare an assay buffer with the following concentrations:; 200 mM Tris-HCl (pH10.0) and 10 mM NAD, 20mM L-proline, and load 150 ul into each well.; 2. Add 50 ul of 1.25 ug and 2.5 ug recombinant PYCR1 protein to each well.; 3. Record the increase in A340nm for 5 minutes at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PYCR1 active protein

PYCR1 is a universal housekeeping enzyme that catalyzes the NAD (P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP (+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). Recombinant human PYCR1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for PYCR1 active protein

Metabolism

References

Kornak u., et al. (2009) Nat Genet. 41 (9):1016-21.; Rao Z., et al (2006) J Mol Biol. 359 (5):1364-77.

NCBI and Uniprot Product Information

• NCBI GI #: 24797097
• NCBI GeneID: 5831
• NCBI Accession #: NP_008838
• NCBI GenBank Nucleotide #: NM_006907.3
• UniProt Accession #: P32322; Q6FHI4; Q96DI6; Q9HBQ4; A6NFM2; B4DMU0
• Molecular Weight: 35.5 kDa (339aa), confirmed by MALDI-TOF
• NCBI Official Full Name: pyrroline-5-carboxylate reductase 1, mitochondrial isoform 1
• NCBI Official Synonym Full Names: pyrroline-5-carboxylate reductase 1
• NCBI Official Symbol: PYCR1
• NCBI Official Synonym Symbols: P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
• NCBI Protein Information: pyrroline-5-carboxylate reductase 1, mitochondrial
• UniProt Protein Name: Pyrroline-5-carboxylate reductase 1, mitochondrial
• Protein Family: Pyrroline-5-carboxylate reductase
• UniProt Gene Name: PYCR1
• UniProt Synonym Gene Names: P5C reductase 1; P5CR 1

NCBI Description

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.