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Western Blot (WB)
(Western blot analysis of RACK1 on different lysates using anti-RACK1 antibody at 1/2, 000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Rat small intestine)
Rabbit RACK1 Monoclonal Antibody | anti-RACK1 antibody
RACK1 Antibody
Gene Names
GNB2L1; H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
RACK1; Monoclonal Antibody; RACK1 Antibody; Cell proliferation-inducing gene 21 protein antibody; GBLP_HUMAN antibody; Gnb2-rs1 antibody; Gnb2l1 antibody; Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody; Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody; Guanine nucleotide binding protein beta subunit 2 like 1 antibody; Guanine nucleotide binding protein beta subunit like protein 12.3 antibody; Guanine nucleotide binding protein subunit beta 2 like 1 antibody; Guanine nucleotide binding protein subunit beta like protein 12.3 antibody; Guanine nucleotide-binding protein subunit beta-2-like 1 antibody; Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody; H12.3 antibody; HLC-7 antibody; Human lung cancer oncogene 7 protein antibody; lung cancer oncogene 7 antibody; OTTHUMP00000223704 antibody; OTTHUMP00000223870 antibody; OTTHUMP00000223891 antibody; OTTHUMP00000223893 antibody; OTTHUMP00000223900 antibody; OTTHUMP00000223902 antibody; OTTHUMP00000223930 antibody; OTTHUMP00000223931 antibody; PIG21 antibody; Proliferation inducing gene 21 antibody; Protein homologous to chicken B complex protein guanine nucleotide binding antibody; RACK1 antibody; Receptor for activated C kinase 1 antibody; Receptor for activated C kinase antibody; Receptor of activated protein kinase C 1 antibody; anti-RACK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JG40-26
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RACK1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein corresponding to C-terminal human RACK1.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of RACK1 on different lysates using anti-RACK1 antibody at 1/2, 000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Rat small intestine)
Western Blot (WB)
(Western blot analysis of RACK1 on different lysates using anti-RACK1 antibody at 1/2, 000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Rat small intestine)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with RACK1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with RACK1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-RACK1 antibody
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. Receptor for activated C kinases, termed RACKs, are intracellular receptors for activated PKC that may be involved in the activation-induced translocation of PKC. RACK1 (receptor for activated C kinase 1) is a 317 amino acid G protein b subunit-like protein that functions as a RACK and inhibits the activity of Src tyrosine kinases. In response to PKC activation, the intracellular localization of RACK1 and PKC bII changes, and RACK1 and PKC bII co-localize to the same sites. RACK1 is therefore thought to be a shuttling protein for PKC bII. A deficit in RACK1 may be associated with impaired PKC activation in the aging brain. The RACK1 gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.pombe, S.cerevisiae, K.lactis, E.gossypii, M.grisea, N.crassa, A.thaliana, rice and P.falciparum, and maps to human chromosome 5q35.3.
Product Categories/Family for anti-RACK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
317
NCBI Official Full Name
guanine nucleotide-binding protein subunit beta-2-like 1
NCBI Official Synonym Full Names
guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
NCBI Official Symbol
GNB2L1
NCBI Official Synonym Symbols
H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
NCBI Protein Information
guanine nucleotide-binding protein subunit beta-2-like 1; lung cancer oncogene 7; proliferation-inducing gene 21; receptor for activated C kinase 1; human lung cancer oncogene 7 protein; receptor of activated protein kinase C 1; cell proliferation-inducin
UniProt Protein Name
Guanine nucleotide-binding protein subunit beta-2-like 1
Protein Family
UniProt Gene Name
GNB2L1
UniProt Synonym Gene Names
HLC-7; RACK1
UniProt Entry Name
GBLP_HUMAN
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Product Notes
The RACK1 gnb2l1 (Catalog #AAA9436704) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RACK1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RACK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the RACK1 gnb2l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RACK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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