Rabbit EF-Tu Polyclonal Antibody | anti-EF-Tu antibody

EF-Tu Polyclonal Antibody

Cat. Num. AAA3011794

• Gene Names: TUFM; P43; EFTU; COXPD4; EF-TuMT
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: EF-Tu; Polyclonal Antibody; EF-Tu Polyclonal Antibody; Elongation factor Tu; mitochondrial; EFTu; P43; TUFM; anti-EF-Tu antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EF-Tu polyclonal antibody detects endogenous levels of EF-Tu protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 455

• Applicable Applications for anti-EF-Tu antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:10-1:100; IP: 1:50-1:200
• Immunogen: Recombinant full length Human EF-Tu.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using TUFM antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using TUFM antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-EF-Tu antibody

EF-Ts, also known as TSFM (Ts translation elongation factor, mitochondrial) or COXPD3, is a 325 amino acid protein that is one of 13 mitochondrial-encoded proteins that work together during the elongation phase of protein biosynthesis on the ribosome. Expressed ubiquitously with highest levels present in liver, kidney and skeletal muscle, EF-Ts associates with EF-Tu, a multidomain GTPase with essential functions in translation, and, via this interaction, facilitates the exchange of GDP for GTP, thereby inducing protein elongation. Mutations in the gene encoding EF-Ts are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3), a condition characterized by defects in the mitochondrial oxidative phosphorylation system and often characterized by severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Multiple isoforms of EF-Ts exist due to alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 34147630
• NCBI GeneID: 7284
• NCBI Accession #: NP_003312.3
• NCBI GenBank Nucleotide #: NP_003312.3
• UniProt Accession #: P49411
• Molecular Weight: ~ 49kDa
• NCBI Official Full Name: elongation factor Tu, mitochondrial isoform 1
• NCBI Official Synonym Full Names: Tu translation elongation factor, mitochondrial
• NCBI Official Symbol: TUFM
• NCBI Official Synonym Symbols: P43; EFTU; COXPD4; EF-TuMT
• NCBI Protein Information: elongation factor Tu, mitochondrial
• UniProt Protein Name: Elongation factor Tu, mitochondrial
• UniProt Gene Name: TUFM
• UniProt Synonym Gene Names: EF-Tu
• UniProt Entry Name: EFTU_HUMAN

NCBI Description

This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

EFTU: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Defects in TUFM are the cause of combined oxidative phosphorylation deficiency type 4 (COXPD4). COXPD4 is characterized by neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Protein type: Mitochondrial; RNA-binding; Translation elongation; Translation

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: mitochondrion; membrane

Molecular Function: GTPase activity; protein binding; GTP binding; translation elongation factor activity

Biological Process: translational elongation; mitochondrial translation; organelle organization and biogenesis

Disease: Combined Oxidative Phosphorylation Deficiency 4