Rabbit Rac1 Polyclonal Antibody

Rac1 (Ras-related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP binding protein Rac1), Cell migration-inducing gene 5 protein, MGC111543, MIG5, p21-Rac1, Ras-like Protein TC25, Ras-related C3 Botulinum Toxin Substrate 1, TC-25)

Cat. Num. AAA628447

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Rac1; Polyclonal Antibody; Rac1 (Ras-related C3 Botulinum Toxin Substrate 1 (Rho Family; Small GTP binding protein Rac1); Cell migration-inducing gene 5 protein; MGC111543; MIG5; p21-Rac1; Ras-like Protein TC25; Ras-related C3 Botulinum Toxin Substrate 1; TC-25); Anti -Rac1 (Ras-related C3 Botulinum Toxin Substrate 1 (Rho Family; anti-Rac1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human Rac1. Does not cross-react with Cdc42. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.15M sodium chloride, pH 7.6, 0.1% sodium azide.

• Applicable Applications for anti-Rac1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500-1:1000
• Immunogen: Synthetic peptide corresponding to aa130-141 of human Rac1 (KEKKLTPITYPQ-C). Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Analysis: L6 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-RAC1 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein RAC1 (~21kD).)

Western Blot (WB)

(Western Blot Analysis: Rat brain lysate with a previous lot of rabbit anti-Rac1. Rac1 has a molecular weight of ~21kD.)

Related Product Information for anti-Rac1 antibody

Rac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers. Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of Rac.

Product Categories/Family for anti-Rac1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 624236
• NCBI Official Full Name: Rac1
• Protein Family: Ras-related protein

Uniprot Description

Involved in axon outgrowth and myoblast fusion. Plays a role in regulating dorsal closure during embryogenesis. Involved in integrin alpha-PS3/beta-nu-mediated phagocytosis of Gram-positive S.aureus by hemocytes.