Chicken Ras-related C3 Botulinum Toxin Substrate 1, Isoform Rac1 Polyclonal Antibody | anti-RAC1 antibody

Ras-related C3 Botulinum Toxin Substrate 1, Isoform Rac1 (Rho Family, Small GTP Binding Protein Rac1)

Cat. Num. AAA610489

• Gene Names: RAC1; MIG5; Rac-1; TC-25; p21-Rac1; MGC111543
• Reactivity: Bovine, Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Ras-related C3 Botulinum Toxin Substrate 1; Isoform Rac1; Polyclonal Antibody; Isoform Rac1 (Rho Family; Small GTP Binding Protein Rac1); Anti -Ras-related C3 Botulinum Toxin Substrate 1; anti-RAC1 antibody

• Host: Chicken
• Reactivity: Bovine, Human, Mouse
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes human Ras-related C3 Botulinum Toxin Substrate 1, Isoform Rac1. Species Crossreactivity: Mouse and bovine.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Glycerol free.

• Applicable Applications for anti-RAC1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:2000
• Immunogen: Synthetic peptide corresponding to aa1-192
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development)

Related Product Information for anti-RAC1 antibody

This protein is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases.

Product Categories/Family for anti-RAC1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 9845511
• NCBI GeneID: 5879
• NCBI Accession #: NP_008839.2
• NCBI GenBank Nucleotide #: NM_006908.4
• UniProt Accession #: P63000; O95501; P15154; Q3Y4D3; Q5JAA8; Q9BTB4; Q59FQ0; AAB22206
• Molecular Weight: 21,450 Da
• NCBI Official Full Name: ras-related C3 botulinum toxin substrate 1 isoform Rac1
• NCBI Official Synonym Full Names: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• NCBI Official Symbol: RAC1
• NCBI Official Synonym Symbols: MIG5; Rac-1; TC-25; p21-Rac1; MGC111543
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 1; OTTHUMP00000151196; OTTHUMP00000151197; ras-like protein TC25; cell migration-inducing gene 5 protein
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 1
• Protein Family: Ras-related protein
• UniProt Gene Name: RAC1
• UniProt Synonym Gene Names: TC25
• UniProt Entry Name: RAC1_HUMAN

NCBI Description

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

RAC1: a plasma membrane-associated member of the Rho-GTPase family. Plays a key role in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. GTP binding stimulates its activity. Phosphorylation by Akt may inhibit GTP binding of Rac1, therefore attenuating the downstream signal transduction pathway. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; G protein; G protein, monomeric; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: Golgi membrane; extrinsic to plasma membrane; focal adhesion; membrane; lamellipodium; cytoplasm; plasma membrane; melanosome; actin filament; trans-Golgi network; cytosol; phagocytic cup

Molecular Function: GTPase activity; protein binding; enzyme binding; GTP binding; GTP-dependent protein binding; Rho GDP-dissociation inhibitor binding; thioesterase binding; protein kinase binding; Rab GTPase binding

Biological Process: axon guidance; viral reproduction; nerve growth factor receptor signaling pathway; metabolic process; positive regulation of apoptosis; cell-matrix adhesion; cell motility involved in cell locomotion; regulation of cell migration; localization within membrane; positive regulation of focal adhesion formation; actin filament polymerization; small GTPase mediated signal transduction; positive regulation of stress fiber formation; response to wounding; ephrin receptor signaling pathway; inflammatory response; cell adhesion; regulation of hydrogen peroxide metabolic process; bone resorption; lamellipodium biogenesis; intercellular junction assembly and maintenance; embryonic olfactory bulb interneuron precursor migration; platelet activation; anatomical structure morphogenesis; Wnt receptor signaling pathway, planar cell polarity pathway; mast cell chemotaxis; dendrite morphogenesis; positive regulation of phosphoinositide 3-kinase activity; positive regulation of Rho protein signal transduction; engulfment of apoptotic cell; regulation of defense response to virus by virus; cell proliferation; G-protein coupled receptor protein signaling pathway; hyperosmotic response; negative regulation of interleukin-23 production; positive regulation of actin filament polymerization; organization of an anatomical structure; T cell costimulation; auditory receptor cell morphogenesis; ruffle organization and biogenesis; innate immune response; cerebral cortex radially oriented cell migration; negative regulation of receptor-mediated endocytosis; positive regulation of protein amino acid phosphorylation; cell motility; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; positive regulation of DNA replication