Rabbit anti-Human RABGAP1 Polyclonal Antibody | anti-RABGAP1 antibody

RABGAP1, NT (RABGAP1, Rab GTPase-activating protein 1, GAP and centrosome-associated protein, Rab6 GTPase-activating protein GAPCenA)

Cat. Num. AAA6007529

• Gene Names: RABGAP1; GAPCENA; TBC1D11; RP11-123N4.2
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RABGAP1; Polyclonal Antibody; NT (RABGAP1; Rab GTPase-activating protein 1; GAP and centrosome-associated protein; Rab6 GTPase-activating protein GAPCenA); Anti -RABGAP1; anti-RABGAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RABGAP1 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Flow Cytometry: 1:10-50
• Immunogen: RABGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-51 amino acids from the N-terminal region of human RABGAP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RABGAP1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RABGAP1 antibody detected the RABGAP1 protein (arrow).)

Flow Cytometry (FC/FACS)

(RABGAP1 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RABGAP1 antibody

RABGAP1 may act as a GTPase-activating protein of RAB6A and play a role in microtubule nucleation by centrosome. It may participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition.

Product Categories/Family for anti-RABGAP1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 32451579
• NCBI GeneID: 23637
• UniProt Accession #: Q9Y3P9; Q05CW2; Q6ZMY1; Q9HA28; Q9P0E2; Q9UG67; B9A6L2
• Molecular Weight: 121,737 Da
• NCBI Official Full Name: RABGAP1 protein
• NCBI Official Synonym Full Names: RAB GTPase activating protein 1
• NCBI Official Symbol: RABGAP1
• NCBI Official Synonym Symbols: GAPCENA; TBC1D11; RP11-123N4.2
• NCBI Protein Information: rab GTPase-activating protein 1; TBC1 domain family, member 11; GAP and centrosome-associated protein; rab6 GTPase-activating protein GAPCenA; Rab6 GTPase activating protein, GAPCenA; rab6 GTPase activating protein (GAP and centrosome-associated)
• UniProt Protein Name: Rab GTPase-activating protein 1
• Protein Family: Rab GTPase-activating protein
• UniProt Gene Name: RABGAP1
• UniProt Entry Name: RBGP1_HUMAN

Uniprot Description

RABGAP1: May act as a GTPase-activating protein of RAB6A. May play a role in microtubule nucleation by centrosome. May participate in a RAB6A-mediated pathway involved in the metaphase- anaphase transition. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; Autophagy; GAPs, Rab

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: centrosome; microtubule associated complex; cytosol

Molecular Function: tubulin binding; protein binding; DNA binding; Rab GTPase binding; GTPase activator activity

Biological Process: cell cycle; positive regulation of GTPase activity