Typical Testing Data/Standard Curve (for reference only)
Rat tissue inhibitors of metalloproteinase 1, TIMP-1 ELISA Kit | TIMP-1 elisa kit
Rat tissue inhibitors of metalloproteinase 1, TIMP-1 ELISA Kit
Reactivity
Rat
Synonyms
tissue inhibitors of metalloproteinase 1; TIMP-1; Rat tissue inhibitors of metalloproteinase 1; TIMP-1 ELISA Kit; TIMP-1 elisa kit
Reactivity
Rat
Sequence Length
207
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 10ng/ml
Sensitivity
0.02ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TIMP-1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Tissue Inhibitors of Metalloproteinase 1 (also known as TIMP-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat TIMP-1 antibody. TIMP-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TIMP-1 Antibody is added and binds to TIMP-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TIMP-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TIMP-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat TIMP-1 antibody. TIMP-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TIMP-1 Antibody is added and binds to TIMP-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TIMP-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TIMP-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,032 Da
NCBI Official Full Name
TIMP-1
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 1
NCBI Official Symbol
TIMP1
NCBI Protein Information
metalloproteinase inhibitor 1
UniProt Protein Name
Metalloproteinase inhibitor 1
UniProt Gene Name
TIMP1
UniProt Synonym Gene Names
EG-1; TIMP-1
Uniprot Description
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
Research Articles on TIMP-1
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Product Notes
The Rat TIMP-1 timp1 (Catalog #AAA161318) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA161318 ELISA Kit recognizes Rat TIMP-1. It is sometimes possible for the material contained within the vial of "tissue inhibitors of metalloproteinase 1, TIMP-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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