Goat anti-Human Rabenosyn 5 Polyclonal Antibody | anti-ZFYVE20 antibody

Rabenosyn 5 (Rabenosyn-5, 110 kD Protein, FLJ34993, FYVE Finger-containing Rab5 Effector Protein Rabenosyn-5, MGC126210, Zinc Finger FYVE Domain-containing Protein 20, ZFYVE20)

Cat. Num. AAA6012599

• Gene Names: ZFYVE20; Rabenosyn-5
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Rabenosyn 5; Polyclonal Antibody; Rabenosyn 5 (Rabenosyn-5; 110 kD Protein; FLJ34993; FYVE Finger-containing Rab5 Effector Protein Rabenosyn-5; MGC126210; Zinc Finger FYVE Domain-containing Protein 20; ZFYVE20); Anti -Rabenosyn 5 (Rabenosyn-5; anti-ZFYVE20 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human ZFYVE20.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-ZFYVE20 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:64,000; Western Blot: 0.3-1.5ug/ml, observed in A431, fibroblast (epidermoid carcinoma) lysates on ~110kD bands
• Immunogen: Synthetic peptide corresponding to C-ELKHTLAKQKGGTD, from human ZFYVE20, at C-terminal (NP_071735.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-ZFYVE20 antibody

DNA binding protein involved in regulation of transcription.

Product Categories/Family for anti-ZFYVE20 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 70980547
• NCBI GeneID: 64145
• NCBI Accession #: NP_071735.2
• NCBI GenBank Nucleotide #: NM_022340.2
• UniProt Accession #: Q9H1K0; Q3KP30; Q59EY8; Q8NAQ1
• Molecular Weight: 88,870 Da
• NCBI Official Full Name: rabenosyn-5
• NCBI Official Synonym Full Names: zinc finger, FYVE domain containing 20
• NCBI Official Symbol: ZFYVE20
• NCBI Official Synonym Symbols: Rabenosyn-5
• NCBI Protein Information: rabenosyn-5; 110 kDa protein; zinc finger FYVE domain-containing protein 20; FYVE finger-containing Rab5 effector protein rabenosyn-5; FYVE-finger-containing Rab5 effector protein rabenosyn-5
• UniProt Protein Name: Rabenosyn-5
• UniProt Gene Name: ZFYVE20
• UniProt Entry Name: RBNS5_HUMAN

NCBI Description

This gene encodes a protein that belongs to the FYVE zinc finger family of proteins. The encoded protein interacts with Ras-related proteins that regulate membrane trafficking. A missense mutation in this gene is associated with a defect in the early endocytic pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

ZFYVE20: Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of CTSD/cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3).

Protein type: Adaptor/scaffold; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: intracellular membrane-bound organelle; early endosome membrane; plasma membrane; endosome membrane; endosome

Molecular Function: protein binding; zinc ion binding; Rab GTPase binding

Biological Process: protein transport; endosomal vesicle fusion; late endosome to vacuole transport; Golgi to endosome transport; Golgi to vacuole transport; endocytosis; vacuole inheritance; blood coagulation; endosome transport