Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse 14-3-3 protein zeta/delta (YWHAZ) Standard Curve.)
Mouse 14-3-3 Protein zeta/delta (YWHAZ) ELISA Kit | YWHAZ elisa kit
Mouse 14-3-3 Protein zeta/delta (YWHAZ) ELISA Kit
Gene Names
Ywhaz; AI596267; AL022924; AU020854; 14-3-3zeta; 1110013I11Rik
Reactivity
Mouse
Synonyms
14-3-3 Protein zeta/delta (YWHAZ); Mouse 14-3-3 Protein zeta/delta (YWHAZ) ELISA Kit; YWHAZ; 14-3-3-zeta; KCIP-1; MGC111427; MGC126532; MGC138156; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; OTTHUMP00000165851; OTTHUMP00000165858; phospholipase A2; protein kinase C inhibitor protein-; YWHAZ elisa kit
Reactivity
Mouse
Specificity
Mouse 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse YWHAZ. No significant cross-reactivity or interference between Mouse YWHAZ and analogues was observed.
Sequence Length
245
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse 14-3-3 protein zeta/delta (YWHAZ) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse 14-3-3 protein zeta/delta (YWHAZ) Standard Curve.)
Related Product Information for YWHAZ elisa kit
Description: This Mouse 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit employs a two-site sandwich ELISA to quantitate YWHAZ in samples. An antibody specific for YWHAZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAZ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: YWHAz belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The deduced 245-amino acid protein shares high sequence homology with other 14-3-3 subtypes. The zeta mRNA was widely expressed in various gray matter brain regions, including the neocortex, hippocampus, caudate-putamen, thalamus, cerebellar cortex, and several brain stem nuclei. A human protein with phospholipase A2 activity was shown to be the zeta subtype of the 14-3-3 protein
Background: YWHAz belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The deduced 245-amino acid protein shares high sequence homology with other 14-3-3 subtypes. The zeta mRNA was widely expressed in various gray matter brain regions, including the neocortex, hippocampus, caudate-putamen, thalamus, cerebellar cortex, and several brain stem nuclei. A human protein with phospholipase A2 activity was shown to be the zeta subtype of the 14-3-3 protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
14-3-3 protein zeta/delta isoform 1
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
NCBI Official Symbol
Ywhaz
NCBI Official Synonym Symbols
AI596267; AL022924; AU020854; 14-3-3zeta; 1110013I11Rik
NCBI Protein Information
14-3-3 protein zeta/delta
UniProt Protein Name
14-3-3 protein zeta/delta
Protein Family
UniProt Gene Name
Ywhaz
UniProt Synonym Gene Names
KCIP-1
UniProt Entry Name
1433Z_MOUSE
Uniprot Description
14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization.
Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold
Cellular Component: extracellular space; mast cell granule; focal adhesion; protein complex; mitochondrion; perinuclear region of cytoplasm; postsynaptic density; cytoplasm; leading edge; nucleus
Molecular Function: protein domain specific binding; identical protein binding; protein binding; protein complex binding; transcription factor binding; protein kinase binding
Biological Process: response to drug; histamine secretion by mast cell; establishment of Golgi localization; protein targeting to mitochondrion; protein targeting
Research Articles on YWHAZ
Similar Products
Product Notes
The Mouse YWHAZ ywhaz (Catalog #AAA9716612) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716612 ELISA Kit recognizes Mouse YWHAZ. It is sometimes possible for the material contained within the vial of "14-3-3 Protein zeta/delta (YWHAZ), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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