Rabbit RAB35 Polyclonal Antibody | anti-RAB35 antibody

RAB35 Polyclonal Antibody

Cat. Num. AAA3006125

• Gene Names: RAB35; RAY; H-ray; RAB1C
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RAB35; Polyclonal Antibody; RAB35 Polyclonal Antibody; Ras-related protein Rab-35; GTP-binding protein RAY; Ras-related protein Rab-1C; RAB1C; RAY; anti-RAB35 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RAB35 polyclonal antibody detects endogenous levels of RAB35 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 152

• Applicable Applications for anti-RAB35 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 146-191 of Human RAB35.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RAB35 pAb at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:L02 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Rat (40ug) Lane4:The Testis tissue lysate of Mouse (40ug))

Related Product Information for anti-RAB35 antibody

The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, all of which are thought to play an important role in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the endoplasmic reticulum (ER) to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Rab 35, also known as Rab 1C or RAY, is a 201 amino acid lipidanchored protein that is thought to function as a GTPase, playing a role in exocytotic and endocytotic pathways and functioning as an important factor in cytokinesis. Rab 35 is expressed ubiquitously and is encoded by a gene that maps to human chromosome 12, which houses over 1, 100 genes and comprises approximately 4.5% of the human genome.

NCBI and Uniprot Product Information

• NCBI GI #: 262527293
• NCBI GeneID: 11021
• NCBI Accession #: NP_001161078.1
• NCBI GenBank Nucleotide #: NP_001161078.1
• UniProt Accession #: Q15286
• Molecular Weight: ~ 23kDa
• NCBI Official Full Name: ras-related protein Rab-35 isoform 2
• NCBI Official Synonym Full Names: RAB35, member RAS oncogene family
• NCBI Official Symbol: RAB35
• NCBI Official Synonym Symbols: RAY; H-ray; RAB1C
• NCBI Protein Information: ras-related protein Rab-35
• UniProt Protein Name: Ras-related protein Rab-35
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB35
• UniProt Synonym Gene Names: RAB1C; RAY
• UniProt Entry Name: RAB35_HUMAN

Uniprot Description

RAB35: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. Interacts with DENND1A and DENND1B in a nucleotide- dependent manner. The low binding to DENND1C is nucleotide- independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Rab; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; intercellular bridge; mitochondrion; cell projection membrane; melanosome; plasma membrane; endosome membrane; coated pit; clathrin-coated endocytic vesicle

Molecular Function: GTPase activity; protein binding; phosphatidylinositol-4,5-bisphosphate binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; antigen processing and presentation; ER to Golgi vesicle-mediated transport; protein localization; metabolic process; cytokinesis; plasma membrane to endosome transport; endosome transport; endocytic recycling; Rab protein signal transduction; neurite development