PEDF recombinant protein

PEDF, 20-418aa, Human, His tag, E Coli

Cat. Num. AAA203343

• Gene Names: GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PEDF; 20-418aa; Human; His tag; E Coli; Pigment epithelium-derived factor; EPC-1; PIG35; SERPINF1; Pigment epithelium-derived factor EPC 1; EPC1; PIG 35; Pigment epithelium derived factor; Proliferation inducing protein 35; SERPIN F1; Serpin peptidase inhibitor clade F member 1; SERPINF 1; ; PEDF recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol.
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP
• Sequence Length: 210

• Applicable Applications for PEDF recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PEDF recombinant protein

PEDF, also known as SERPINF1, is an extracellular neurotrophic agent, first identified as a secreted product in conditioned medium from cultured fetal human retinal pigment epithelial (RPE) cells. It was reported that PEDF isolated from medium conditioned by human fetal RPE primary cultures promotes neurite outgrowth in cultured human retinoblastoma Y-79 cells. PEDF is also a member of the serpin family, a group of serine protease inhibitors. As PEDF does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Recombinant PEDF protein containing a natural variant M72T was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PEDF recombinant protein

Angiogenesis

References

Steele FR., et al. (1993) Proc Natl Acad Sci u S A. 90 (4):1526-30..; Becerra SP., et al. (1995) J Biol Chem. 270 (43):25992-9.;

NCBI and Uniprot Product Information

• NCBI GI #: 4504183
• NCBI GeneID: 2950
• NCBI Accession #: NP_000843
• NCBI GenBank Nucleotide #: NM_000852.3
• UniProt Accession #: P09211; O00460; Q15690; Q5TZY3
• Molecular Weight: 46.7 kDa (420 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: glutathione S-transferase P
• NCBI Official Synonym Full Names: glutathione S-transferase pi 1
• NCBI Official Symbol: GSTP1
• NCBI Official Synonym Symbols: PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
• NCBI Protein Information: glutathione S-transferase P
• UniProt Protein Name: Glutathione S-transferase P
• UniProt Gene Name: GSTP1
• UniProt Synonym Gene Names: FAEES3; GST3

NCBI Description

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.