Western Blot (WB)
(Anti-RAB1A rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: RT-4 Whole Cell Lysate Lane D: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:25 kDa)
Rabbit anti-Human RAB1A Polyclonal Antibody | anti-RAB1A antibody
Anti-RAB1A Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
RAB1A; Polyclonal Antibody; Anti-RAB1A Antibody; Rabbit Polyclonal; RAB1A Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-RAB1 Antibody; Anti-YPT1 Antibody; member RAS oncogene family; anti-RAB1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human RAB1A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-RAB1A antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-5uL/mg of Lysate
IP: 1-5uL/mg of Lysate
Immunogen
E Coli-derived Human RAB1A fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human RAB1A fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Western Blot (WB)
(Anti-RAB1A rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: RT-4 Whole Cell Lysate Lane D: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:25 kDa)
Western Blot (WB)
(Anti-RAB1A rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: RT-4 Whole Cell Lysate Lane D: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:25 kDa)
Immunoprecipitation (IP)
(RAB1A was immunoprecipitated using: Lane A:0.5 mg RT-4 Whole Cell Lysate 4 uL anti-RAB1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RAB1A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa Observed band size :26 kDa)
Immunoprecipitation (IP)
(RAB1A was immunoprecipitated using: Lane A:0.5 mg RT-4 Whole Cell Lysate 4 uL anti-RAB1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RAB1A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa Observed band size :26 kDa)
Related Product Information for anti-RAB1A antibody
Background: Rab1A is a newly identified mTORC1 activator that mediates an alternative amino acid (AA) signaling branch to Rag GTPases. Rab1a is a member of the Rab family of small GTPases with a well-characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. RAB1A acts as an oncogene in various cancers, and emerging evidence has verified that RAB1A is a mTORC1 activator in hepatocellular and colorectal cancer. RAB1A acts as an oncogene by regulating cellular proliferation, growth, invasion, and metastasis via activation of the mTORC1 pathway in triple-negative breast cancer. RAB1A is an important small molecule in the mechanistic target of the rapamycin signaling pathway, which is one of the downstream signaling pathways of the epidermal growth factor receptor family.
Product Categories/Family for anti-RAB1A antibody
References
Xu H, et al. (2017) Inhibition of rab1a suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells. Oncol Rep 37 (3): 1619-1626.Xu B, et al. (2018) Significance and prognostic role of human epidermal growth factor receptor 2 and rab1a expression in gastric cancer. Oncol Lett 15 (4): 5185-5192.Wang C, et al. (2010) Regulation of integrin beta 1 recycling to lipid rafts by rab1a to promote cell migration. J Biol Chem 285 (38): 29398-29405.
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Product Notes
The RAB1A (Catalog #AAA8119337) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAB1A Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAB1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of Lysate. Researchers should empirically determine the suitability of the RAB1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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