Rabbit PZR Polyclonal Antibody | anti-MPZL1 antibody

PZR, Phosphorylated (Tyr263) (Myelin Protein Zero-like Protein 1, Protein Zero-related, MPZL1, UNQ849/PRO1787)

Cat. Num. AAA645845

• Gene Names: MPZL1; PZR; PZRa; PZRb; PZR1b; MPZL1b
• Reactivity: Bovine, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PZR; Polyclonal Antibody; Phosphorylated (Tyr263) (Myelin Protein Zero-like Protein 1; Protein Zero-related; MPZL1; UNQ849/PRO1787); Anti -PZR; anti-MPZL1 antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of human PZR when phosphorylated at Tyr263. Species Crossreactivity: Bovine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-MPZL1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide corresponding to residues surrounding Tyr263 of human PZR.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from BAEC cells, untreated or H2O2-treated (0.3mM for 30min), using MBS645845 and total PZR (lower).)

Related Product Information for anti-MPZL1 antibody

PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is a main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. Recently, PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan Syndrome (8).

Product Categories/Family for anti-MPZL1 antibody

Antibodies; Abs to Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 3851145
• NCBI GeneID: 9019
• UniProt Accession #: O95297; Q5R332; Q8IX11; Q9BWZ3; Q9NYK4; Q9UL20; B2REB9; B2REC0
• Molecular Weight: 29,082 Da
• NCBI Official Full Name: protein zero related protein
• NCBI Official Synonym Full Names: myelin protein zero-like 1
• NCBI Official Symbol: MPZL1
• NCBI Official Synonym Symbols: PZR; PZRa; PZRb; PZR1b; MPZL1b
• NCBI Protein Information: myelin protein zero-like protein 1; protein zero related; protein zero-related; immunoglobulin family transmembrane protein
• UniProt Protein Name: Myelin protein zero-like protein 1
• Protein Family: Myelin protein zero-like protein
• UniProt Gene Name: MPZL1
• UniProt Synonym Gene Names: PZR
• UniProt Entry Name: MPZL1_HUMAN

Uniprot Description

Function: Cell surface receptor, which is involved in signal transduction processes. Recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. Is a major receptor for concanavalin-A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine-protein kinases. Isoform 3 seems to have a dominant negative role; it blocks tyrosine phosphorylation of MPZL1 induced by ConA. Isoform 1, but not isoform 2 and isoform 3, may be involved in regulation of integrin-mediated cell motility. Ref.3 Ref.4 Ref.12

Subunit structure: Interacts with phosphorylated PTPN11/SHP-2. Ref.1 Ref.11

Subcellular location: Membrane; Single-pass type I membrane protein

Potential.

Tissue specificity: Widely expressed with highest levels in heart, placenta, kidney and pancreas. Isoform 3 is relatively abundant in hematopoietic tissues and fetal liver. Isoform 1 and isoform 3 are expressed in CD14- PB monocytes and pre-B cell progenitors. Isoform 3 appears to be the major isoform in CD34- promyelocytic and promonocytic cells. During differentiation in monocytic cells, the expression level of isoform 3 decreases and that of isoform 1 increases. Isoform 1 is prominent in stromal cells and, to a lesser extent, in umbilical vein endothelial cells and erythroid progenitors. Isoform 2 is expressed in a erythroid progenitor cell line. Ref.1 Ref.3 Ref.4

Domain: Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Post-translational modification: Phosphorylated on tyrosine residues upon stimulation with pervanadate and concanavalin-A (ConA). Phosphorylation at Tyr-241 and Tyr-263 is required for interaction with PTPN11/SHP-2. Dephosphorylated by PTPN11/SHP-2 (in vitro). Ref.1 Ref.11 Ref.12N-glycosylated. Ref.1 Ref.12

Sequence similarities: Belongs to the myelin P0 protein family.Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Sequence caution: The sequence CAI20144.2 differs from that shown. Reason: Erroneous gene model prediction.