Western Blot (WB)
(Western blot analysis of Pyridoxal Kinase expression in HT29 (A), Hela (B), mouse brain (C), rat liver (D) whole cell lysates.)
Rabbit anti-Human Pyridoxal Kinase Polyclonal Antibody | anti-PDXK antibody
Anti-Pyridoxal Kinase Antibody
Western Blot (WB)
(Western blot analysis of Pyridoxal Kinase expression in HT29 (A), Hela (B), mouse brain (C), rat liver (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Pyridoxal Kinase expression in HT29 (A), Hela (B), mouse brain (C), rat liver (D) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Pyridoxal Kinase staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Pyridoxal Kinase staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
PDXK: Required for synthesis of pyridoxal-5-phosphate from vitamin B6. Belongs to the pyridoxine kinase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, other; Cofactor and Vitamin Metabolism - vitamin B6; EC 2.7.1.35
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus
Molecular Function: ATP binding; lithium ion binding; magnesium ion binding; potassium ion binding; protein homodimerization activity; pyridoxal kinase activity; pyridoxal phosphate binding; sodium ion binding; zinc ion binding
Biological Process: cell proliferation; phosphorylation; pyridoxal 5'-phosphate salvage; pyridoxal phosphate biosynthetic process; vitamin B6 metabolic process
Research Articles on PDXK
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Product Notes
The PDXK pdxk (Catalog #AAA8245622) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Pyridoxal Kinase Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pyridoxal Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/10 - 1/100. Researchers should empirically determine the suitability of the PDXK pdxk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyridoxal Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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