Typical Testing Data/Standard Curve (for reference only)
Human IFN-alpha ELISA Kit | IFN-alpha elisa kit
Human IFN-alpha (Interferon Alpha) ELISA Kit
Gene Names
IFNA1; IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
Reactivity
Human
Synonyms
IFN-alpha; Human IFN-alpha (Interferon Alpha) ELISA Kit; IFN-alpha elisa kit
Reactivity
Human
Specificity
This kit recognizes Human IFNalpha1 in samples. No significant cross-reactivity or interference between Human IFNalpha1 and analogues was observed.
Sequence Length
189
Samples
Serum and plasma, please inquire if your samples are other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
1.88-200pg/mL
Sensitivity
3.13pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IFNalpha1 were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IFNalpha1 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IFN-alpha elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IFNalpha1 concentrations in serum and plasma, please inquire if your samples are other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFNalpha1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFNalpha1 and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFNalpha1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Human IFNalpha1. You can calculate the concentration of Human IFNalpha1 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFNalpha1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFNalpha1 and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFNalpha1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Human IFNalpha1. You can calculate the concentration of Human IFNalpha1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,725 Da
NCBI Official Full Name
interferon alpha-1/13
NCBI Official Synonym Full Names
interferon, alpha 1
NCBI Official Symbol
IFNA1
NCBI Official Synonym Symbols
IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
NCBI Protein Information
interferon alpha-1/13; IFN-alpha 1b; IFN-alpha-1/13; interferon alpha 1b; interferon alpha-D; interferon-alpha1; leIF D
UniProt Protein Name
Interferon alpha-1/13
UniProt Gene Name
IFNA1
UniProt Synonym Gene Names
IFN-alpha-1/13; LeIF D
UniProt Entry Name
IFNA1_HUMAN
NCBI Description
The protein encoded by this gene is produced by macrophages and has antiviral activity. This gene is intronless and the encoded protein is secreted. [provided by RefSeq, Sep 2011]
Uniprot Description
IFNA1/13: a cytokine that belongs to the alpha/beta interferon family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: extracellular space; extracellular region
Molecular Function: interferon-alpha/beta receptor binding; cytokine activity
Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; humoral immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; T cell activation during immune response
Research Articles on IFN-alpha
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Product Notes
The Human IFN-alpha ifna1 (Catalog #AAA2506739) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2506739 ELISA Kit recognizes Human IFN-alpha. It is sometimes possible for the material contained within the vial of "IFN-alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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