Human Pyridoxal kinase ELISA Kit | PDXK elisa kit

Human Pyridoxal kinase ELISA Kit

Cat. Num. AAA763269

• Gene Names: PDXK; PKH; PNK; PRED79; C21orf97; HEL-S-1a; C21orf124
• Reactivity: Human
• Synonyms: Pyridoxal kinase; Human Pyridoxal kinase ELISA Kit; PDXK/Pyridoxal kinase/Pyridoxine kinase/PRED79/C21orf124/C21orf97/PKH/PNK/Vitamin B6 Kinase/HEL-S-1a; PDXK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PDGFRb. No significant cross-reactivity or interference between PDGFRb and analogues was observed.
• Sequence Length: 312

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDGFRb were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDGFRb were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDXK elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PDGFRb antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PDGFRb antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PDGFRb amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PDGFRb can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4505701
• NCBI GeneID: 8566
• NCBI Accession #: NP_003672.1
• NCBI GenBank Nucleotide #: NM_003681.4
• Molecular Weight: 26,827 Da
• NCBI Official Full Name: pyridoxal kinase
• NCBI Official Synonym Full Names: pyridoxal (pyridoxine, vitamin B6) kinase
• NCBI Official Symbol: PDXK
• NCBI Official Synonym Symbols: PKH; PNK; PRED79; C21orf97; HEL-S-1a; C21orf124
• NCBI Protein Information: pyridoxal kinase
• UniProt Protein Name: Pyridoxal kinase
• Protein Family: Pyridoxal kinase
• UniProt Gene Name: PDXK
• UniProt Synonym Gene Names: C21orf124; C21orf97; PKH; PNK
• UniProt Entry Name: PDXK_HUMAN

NCBI Description

The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PDXK: Required for synthesis of pyridoxal-5-phosphate from vitamin B6. Belongs to the pyridoxine kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; Cofactor and Vitamin Metabolism - vitamin B6; EC 2.7.1.35

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: ATP binding; lithium ion binding; magnesium ion binding; potassium ion binding; protein homodimerization activity; pyridoxal kinase activity; pyridoxal phosphate binding; sodium ion binding; zinc ion binding

Biological Process: cell proliferation; phosphorylation; pyridoxal 5'-phosphate salvage; pyridoxal phosphate biosynthetic process; vitamin B6 metabolic process; vitamin metabolic process; water-soluble vitamin metabolic process