Rabbit anti-Human PYCARD Polyclonal Antibody | anti-PYCARD antibody

PYCARD, CT (PYCARD, ASC, CARD5, TMS1, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, Target of methylation-induced silencing 1) (Azide free) (HRP)

Cat. Num. AAA6339320

• Gene Names: PYCARD; ASC; TMS; TMS1; CARD5; TMS-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PYCARD; Polyclonal Antibody; CT (PYCARD; ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1) (Azide free) (HRP); Target of methylation-induced silencing 1; anti-PYCARD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PYCARD
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PYCARD antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 129-158 in the C-terminal region of human PYCARD, UniProt Accession #NP_037390.2; NP_660183.1; Q9ULZ3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PYCARD Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PYCARD antibody detected the PYCARD protein (arrow).)

Product Categories/Family for anti-PYCARD antibody

Antibodies; Apoptosis

References

1. Grau, E., et al. J. Cancer Res. Clin. Oncol. 136(9):1415-1421(2010). 2. Motani, K., et al. Cancer Sci. 101(8):1822-1827(2010). 3. Mishra, B.B., et al. Cell. Microbiol. 12(8):1046-1063(2010). 4. Cheng, J., et al. J. Cell. Physiol. 222(3):738-747(2010). 5. McElvania Tekippe, E., et al. PLoS ONE 5 (8), E12320 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 10835256
• NCBI GeneID: 29108
• NCBI Accession #: NP_037390.2; NP_660183.1
• NCBI GenBank Nucleotide #: NM_013258.4
• UniProt Accession #: Q9ULZ3; Q96D12; Q9BSZ5; Q9HBD0; Q9NXJ8
• Molecular Weight: 21627 MW
• NCBI Official Full Name: apoptosis-associated speck-like protein containing a CARD isoform a
• NCBI Official Synonym Full Names: PYD and CARD domain containing
• NCBI Official Symbol: PYCARD
• NCBI Official Synonym Symbols: ASC; TMS; TMS1; CARD5; TMS-1
• NCBI Protein Information: apoptosis-associated speck-like protein containing a CARD
• UniProt Protein Name: Apoptosis-associated speck-like protein containing a CARD
• Protein Family: Apoptosis-associated speck-like protein
• UniProt Gene Name: PYCARD
• UniProt Synonym Gene Names: ASC; CARD5; TMS1; hASC
• UniProt Entry Name: ASC_HUMAN

NCBI Description

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell soma; mitochondrion; endoplasmic reticulum; cytoplasm; nucleolus; extracellular region; IkappaB kinase complex; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protease binding; caspase activator activity; Pyrin domain binding

Biological Process: myeloid dendritic cell activation during immune response; apoptosis; positive regulation of apoptosis; regulation of protein stability; positive regulation of caspase activity; positive regulation of JNK cascade; positive regulation of interleukin-1 beta secretion; positive regulation of activated T cell proliferation; signal transduction; defense response to Gram-negative bacterium; activation of NF-kappaB transcription factor; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; inflammatory response; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus; positive regulation of adaptive immune response; caspase activation; interleukin-1 beta production; positive regulation of interleukin-6 production; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of tumor necrosis factor production; negative regulation of interferon-beta production; myeloid dendritic cell activation; positive regulation of interferon-gamma production; positive regulation of actin filament polymerization; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; innate immune response; regulation of autophagy; positive regulation of transcription factor activity; positive regulation of T cell activation; positive regulation of defense response to virus by host; activation of innate immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II