Endothelin 1 (EDN1) Recombinant Protein | EDN1 recombinant protein

Recombinant Endothelin 1 (EDN1)

Cat. Num. AAA2018815

• Gene Names: Edn1; ET-1; preproET
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Endothelin 1 (EDN1); Recombinant Endothelin 1 (EDN1); EDN1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF- ENGVQSPP PSTPWRPRRS KRCSCSSLMD KECVYFCHLD IIWVNTPERV VPYGLGGSSR SKRSLKDLLP NKATDQAVRC QCAHQKDKKC WNFCQAGKEL RAQSTMQKSL KDSKKGKPCS KLGKKCIYQQ LVEGRKLRRL EAISNSIKAS FRVAKLKAELYRDQKLTHNR AH
• Sequence Length: 202

• Applicable Applications for EDN1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Glu33~His202 (Accession # P22387) with N-terminal His-Tag
• Subcellular Location: Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9.9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 6753720
• NCBI GeneID: 13614
• NCBI Accession #: NP_034234.1
• NCBI GenBank Nucleotide #: NM_010104.3
• UniProt Accession #: P22387; P70203; P97338
• Molecular Weight: 21.0kDa
• NCBI Official Full Name: endothelin-1
• NCBI Official Synonym Full Names: endothelin 1
• NCBI Official Symbol: Edn1
• NCBI Official Synonym Symbols: ET-1; preproET
• NCBI Protein Information: endothelin-1; PPET1; preproendothelin; preproendothelin-1
• UniProt Protein Name: Endothelin-1
• Protein Family: Endothelin
• UniProt Gene Name: Edn1
• UniProt Synonym Gene Names: ET-1; PPET1
• UniProt Entry Name: EDN1_MOUSE

Uniprot Description

EDN1: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell; cytoplasm; extracellular region; cytosol

Molecular Function: protein binding; hormone activity; endothelin B receptor binding; cytokine activity; endothelin A receptor binding; receptor binding

Biological Process: positive regulation of JNK activity; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of systemic arterial blood pressure by endothelin; heart development; middle ear morphogenesis; prostaglandin biosynthetic process; sensory perception of pain; positive regulation of MAP kinase activity; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; cell surface receptor linked signal transduction; cell-cell signaling; negative regulation of nitric-oxide synthase biosynthetic process; protein kinase C activation; regulation of blood vessel size; cell growth; rhythmic excitation; positive regulation of mitosis; positive regulation of heart rate; respiratory gaseous exchange; peptide hormone secretion; patterning of blood vessels; membrane depolarization; protein kinase C deactivation; regulation of vasoconstriction; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; superoxide release; positive regulation of odontogenesis; epithelial fluid transport; phosphoinositide 3-kinase cascade; G-protein signaling, phospholipase D activating pathway; positive regulation of cell size; neural crest cell development; negative regulation of hormone secretion; positive regulation of smooth muscle cell proliferation; negative regulation of cellular protein metabolic process; glucose transport; negative regulation of transcription from RNA polymerase II promoter; vein smooth muscle contraction; histamine secretion; nitric oxide transport; regulation of blood pressure; positive regulation of cell proliferation; regulation of pH; positive regulation of smooth muscle contraction; skeletal development; artery smooth muscle contraction; vasoconstriction; inositol phosphate-mediated signaling; in utero embryonic development; calcium-mediated signaling; positive regulation of hormone secretion; body fluid secretion; G-protein coupled receptor protein signaling pathway; dorsal/ventral pattern formation; cartilage development; positive regulation of prostaglandin secretion; response to hypoxia; regulation of sensory perception of pain; positive regulation of cell migration