Human Lamin B2 (LMNB2) ELISA Kit | LMNB2 elisa kit

Human Lamin B2 (LMNB2) ELISA Kit

Cat. Num. AAA2023667

• Gene Names: LMNB2; LMN2; LAMB2
• Reactivity: Human
• Synonyms: Lamin B2 (LMNB2); Human Lamin B2 (LMNB2) ELISA Kit; LMN2; LAMB2; LMNB2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lamin B2 (LMNB2).; No significant cross-reactivity or interference between Lamin B2 (LMNB2) and analogues was observed.
• Sequence Length: 600

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.051ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lamin B2 (LMNB2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lamin B2 (LMNB2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LMNB2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lamin B2 (LMNB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lamin B2 (LMNB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lamin B2 (LMNB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lamin B2 (LMNB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LMNB2 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 1054874
• NCBI GeneID: 84823
• UniProt Accession #: Q03252; O75292; Q14734; Q96DF6
• Molecular Weight: 67,689 Da
• NCBI Official Full Name: lamin B2, partial
• NCBI Official Synonym Full Names: lamin B2
• NCBI Official Symbol: LMNB2
• NCBI Official Synonym Symbols: LMN2; LAMB2
• NCBI Protein Information: lamin-B2
• UniProt Protein Name: Lamin-B2
• Protein Family: Lamin
• UniProt Gene Name: LMNB2
• UniProt Synonym Gene Names: LMN2
• UniProt Entry Name: LMNB2_HUMAN

NCBI Description

This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy. [provided by RefSeq, May 2012]

Uniprot Description

Lamin B2: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Lamin B2 may be lost during the follicle centre stage of B-cell differentiation in humans. Nuclear lamins are cleaved by caspases during apoptosis.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: lamin filament; nuclear inner membrane

Molecular Function: structural molecule activity

Disease: Lipodystrophy, Partial, Acquired, Susceptibility To