Rabbit anti-Human PTRH2 Polyclonal Antibody | anti-PTRH2 antibody

PTRH2, NT (PTRH2, BIT1, PTH2, Peptidyl-tRNA hydrolase 2, mitochondrial, Bcl-2 inhibitor of transcription 1) (Biotin)

Cat. Num. AAA6339120

• Gene Names: PTRH2; BIT1; PTH2; CFAP37; CGI-147
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTRH2; Polyclonal Antibody; NT (PTRH2; BIT1; PTH2; Peptidyl-tRNA hydrolase 2; mitochondrial; Bcl-2 inhibitor of transcription 1) (Biotin); Bcl-2 inhibitor of transcription 1; anti-PTRH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PTRH2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PTRH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human PTRH2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PTRH2 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PTRH2 antibody detected the PTRH2 protein (arrow).)

Related Product Information for anti-PTRH2 antibody

The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis (By similarity). Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.

Product Categories/Family for anti-PTRH2 antibody

Antibodies; Mitochondrial Proteins

References

Fan, T., et al. Int. J. Oncol. 35(2):359-367(2009). Biliran, H., et al. J. Biol. Chem. 283(42):28029-28037(2008). Bouchentouf, M., et al. Am. J. Transplant. 7(6):1491-1505(2007). Jan, Y., et al. Cell 116(5):751-762(2004). De Pereda, J.M., et al. J. Biol. Chem. 279(9):8111-8115(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 7706351
• NCBI GeneID: 51651
• NCBI Accession #: NP_057161.1
• NCBI GenBank Nucleotide #: NM_016077.3
• UniProt Accession #: Q9Y3E5; Q9NTE5; B3KUY4
• Molecular Weight: 19,194 Da
• NCBI Official Full Name: peptidyl-tRNA hydrolase 2, mitochondrial
• NCBI Official Synonym Full Names: peptidyl-tRNA hydrolase 2
• NCBI Official Symbol: PTRH2
• NCBI Official Synonym Symbols: BIT1; PTH2; CFAP37; CGI-147
• NCBI Protein Information: peptidyl-tRNA hydrolase 2, mitochondrial; PTH 2; bcl-2 inhibitor of transcription 1; cilia and flagella associated protein 37
• UniProt Protein Name: Peptidyl-tRNA hydrolase 2, mitochondrial
• UniProt Gene Name: PTRH2
• UniProt Synonym Gene Names: BIT1; PTH2; PTH 2
• UniProt Entry Name: PTH2_HUMAN

NCBI Description

The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators. This gene has been implicated in the development and progression of tumors, and mutations in this gene have been associated with an infantile multisystem neurologic, endocrine, and pancreatic disease (INMEPD) characterized by intellectual disability, postnatal microcephaly, progressive cerebellar atrophy, hearing impairment, polyneuropathy, failure to thrive, and organ fibrosis with exocrine pancreas insufficiency (PMID: 25574476). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Uniprot Description

PTRH2: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis. Monomer. Belongs to the PTH2 family.

Protein type: EC 3.1.1.29; Mitochondrial; Hydrolase

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: membrane; mitochondrion; cytosol

Molecular Function: protein binding; aminoacyl-tRNA hydrolase activity

Biological Process: apoptosis; metabolic process

Disease: Neurologic, Endocrine, And Pancreatic Disease, Multisystem, Infantile-onset