PTRH2 recombinant protein

PTRH2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121488

• Gene Names: PTRH2; BIT1; PTH2; CFAP37; CGI-147
• Purity: >95% as determined by SDS-PAGE
• Synonyms: PTRH2; PTRH2 Protein; Human; Recombinant (His Tag); Human PTH2/PTRH2 Protein (His Tag); BIT1 Protein; CFAP37 Protein; CGI-147 Protein; IMNEPD Protein; PTH Protein; PTH 2 Protein; PTH2 Protein; peptidyl-tRNA hydrolase 2; PTRH2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly63-Tyr179

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human PTRH2 (Q9Y3E5)(Gly63-Tyr179) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PTRH2 recombinant protein

Background: PTH2, also known as PTRH2, is a mitochondrial protein that belongs to the PTH2 family. PTH 2 is released during apoptosis from the mitochondria to the cytoplasm. The natural substrate for PTH 2 may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. When in the cytoplasm, PTRH2 regulates the function of 2 transcriptional regulators, TLE5 and TLE1, thus promoting caspase-independent cell death.

References

Prahalad AK. et al., 2006, Proteomics. 6 (12): 3482-93. Jin L. et al., 2000, J Biol Chem. 275 (35): 27238-44. Boone T. et al., 2000, Nat Struct Biol. 7 (6): 486-91.

NCBI and Uniprot Product Information

• NCBI GI #: 7706351
• NCBI GeneID: 51651
• NCBI Accession #: NP_057161.1
• NCBI GenBank Nucleotide #: NM_016077.3
• UniProt Accession #: Q9Y3E5; Q9NTE5; B3KUY4
• Molecular Weight: 19,194 Da
• NCBI Official Full Name: peptidyl-tRNA hydrolase 2, mitochondrial
• NCBI Official Synonym Full Names: peptidyl-tRNA hydrolase 2
• NCBI Official Symbol: PTRH2
• NCBI Official Synonym Symbols: BIT1; PTH2; CFAP37; CGI-147
• NCBI Protein Information: peptidyl-tRNA hydrolase 2, mitochondrial; PTH 2; bcl-2 inhibitor of transcription 1; cilia and flagella associated protein 37
• UniProt Protein Name: Peptidyl-tRNA hydrolase 2, mitochondrial
• UniProt Gene Name: PTRH2
• UniProt Synonym Gene Names: BIT1; PTH2; PTH 2
• UniProt Entry Name: PTH2_HUMAN

NCBI Description

The protein encoded by this gene is a mitochondrial protein with two putative domains, an N-terminal mitochondrial localization sequence, and a UPF0099 domain. In vitro assays suggest that this protein possesses peptidyl-tRNA hydrolase activity, to release the peptidyl moiety from tRNA, thereby preventing the accumulation of dissociated peptidyl-tRNA that could reduce the efficiency of translation. This protein also plays a role regulating cell survival and death. It promotes survival as part of an integrin-signaling pathway for cells attached to the extracellular matrix (ECM), but also promotes apoptosis in cells that have lost their attachment to the ECM, a process called anoikos. After loss of cell attachment to the ECM, this protein is phosphorylated, is released from the mitochondria into the cytosol, and promotes caspase-independent apoptosis through interactions with transcriptional regulators. This gene has been implicated in the development and progression of tumors, and mutations in this gene have been associated with an infantile multisystem neurologic, endocrine, and pancreatic disease (INMEPD) characterized by intellectual disability, postnatal microcephaly, progressive cerebellar atrophy, hearing impairment, polyneuropathy, failure to thrive, and organ fibrosis with exocrine pancreas insufficiency (PMID: 25574476). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Uniprot Description

PTRH2: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis. Monomer. Belongs to the PTH2 family.

Protein type: EC 3.1.1.29; Mitochondrial; Hydrolase

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: membrane; mitochondrion; cytosol

Molecular Function: protein binding; aminoacyl-tRNA hydrolase activity

Biological Process: apoptosis; metabolic process

Disease: Neurologic, Endocrine, And Pancreatic Disease, Multisystem, Infantile-onset