Rabbit anti-Human KYNU Polyclonal Antibody | anti-KYNU antibody

KYNU, CT (Kynureninase, L-kynurenine Hydrolase)

Cat. Num. AAA625446

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: KYNU; Polyclonal Antibody; CT (Kynureninase; L-kynurenine Hydrolase); Anti -KYNU; anti-KYNU antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KYNU.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-KYNU antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human KYNU (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KYNU Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). KYNU (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KYNU Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(KYNU Antibody (C-term) flow cytometry analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KYNU antibody

KYNU is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. KYNU is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway.

Product Categories/Family for anti-KYNU antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 48146963
• NCBI GeneID: 8942
• UniProt Accession #: Q16719; Q6I9T2; Q9BVW3; B2RCZ5; D3DP79
• Molecular Weight: 52,352 Da
• NCBI Official Full Name: KYNU
• NCBI Official Synonym Full Names: kynureninase
• NCBI Official Symbol: KYNU
• NCBI Protein Information: kynureninase; kynureninase (L-kynurenine hydrolase)
• UniProt Protein Name: Kynureninase
• Protein Family: Kynureninase
• UniProt Gene Name: KYNU
• UniProt Entry Name: KYNU_HUMAN

NCBI Description

Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Uniprot Description

KYNU: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity. Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively. Belongs to the kynureninase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; EC 3.7.1.3; Mitochondrial; Hydrolase

Chromosomal Location of Human Ortholog: 2q22.2

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol

Molecular Function: protein homodimerization activity; kynureninase activity; pyridoxal phosphate binding

Biological Process: anthranilate metabolic process; tryptophan catabolic process to acetyl-CoA; tryptophan catabolic process to kynurenine; quinolinate biosynthetic process; tryptophan catabolic process

Disease: Hydroxykynureninuria