Rabbit anti-Human HMG2 Polyclonal Antibody | anti-HMG2 antibody

HMG2 (High-mobility Group Box 2, High Mobility Group Protein B2, High Mobility Group Protein 2, HMG2, HMG-2, HMGB2) (AP)

Cat. Num. AAA6480986

• Gene Names: HMGB2; HMG2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: HMG2; Polyclonal Antibody; HMG2 (High-mobility Group Box 2; High Mobility Group Protein B2; High Mobility Group Protein 2; HMG-2; HMGB2) (AP); HMG Transcription Factor Box2; anti-HMG2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HMGB2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-HMG2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human HMGB2 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HMGB2 Antibody (Center) western blot analysis in HL-60,K562,H-4-II-E cell line lysates (35ug/lane).This demonstrates the HMGB2 antibody detected the HMGB2 protein (arrow).)

Product Categories/Family for anti-HMG2 antibody

Antibodies; Transcription Factors

References

1. Majumdar A., Brown D. 19:6643-6643(1991). 2. Shirakawa H., Yoshida M.J. 267:6641-6645(1992). 3. Alexandre S., Li W.W.Nucleic Acids Res. 20:6413-6413(1992). 4. Fan Z., Beresford P.J.Mol. Cell. Biol. 22:2810-2820(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 11321591
• NCBI GeneID: 3148
• NCBI Accession #: NP_002120
• NCBI GenBank Nucleotide #: NM_002129.3
• UniProt Accession #: P26583; Q5U072; B2R4K8; D3DP37
• Molecular Weight: 26.4 kDa (232aa)
• NCBI Official Full Name: high mobility group protein B2
• NCBI Official Synonym Full Names: high mobility group box 2
• NCBI Official Symbol: HMGB2
• NCBI Official Synonym Symbols: HMG2
• NCBI Protein Information: high mobility group protein B2
• UniProt Protein Name: High mobility group protein B2
• Protein Family: HMG2-induced ER-remodeling protein
• UniProt Gene Name: HMGB2
• UniProt Synonym Gene Names: HMG2; HMG-2
• UniProt Entry Name: HMGB2_HUMAN

NCBI Description

This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq, Jul 2008]

Uniprot Description

HMGB2: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Belongs to the HMGB family.

Protein type: DNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 4q31

Cellular Component: nucleoplasm; extracellular space; protein complex; perinuclear region of cytoplasm; cytoplasm; condensed chromosome; nucleus

Molecular Function: protein domain specific binding; RAGE receptor binding; protein binding; DNA binding; double-stranded DNA binding; damaged DNA binding; chromatin binding; DNA bending activity; transcription factor activity; single-stranded DNA binding; chemoattractant activity

Biological Process: positive regulation of nuclease activity; establishment and/or maintenance of chromatin architecture; DNA topological change; V(D)J recombination; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of erythrocyte differentiation; male gonad development; spermatid nuclear differentiation; base-excision repair, DNA ligation; regulation of transcription from RNA polymerase II promoter; chromatin remodeling; nucleosome assembly; positive chemotaxis; response to steroid hormone stimulus; DNA ligation during DNA repair; positive regulation of megakaryocyte differentiation; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; negative regulation of transcription, DNA-dependent; positive regulation of DNA binding; cell structure disassembly during apoptosis