Mouse anti-Human CD18 Monoclonal Antibody | anti-CD18 antibody

Anti-Hu CD18 Alexa Fluor700

• Gene Names: ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD18; Monoclonal Antibody; Anti-Hu CD18 Alexa Fluor700; Integrin beta2; Complement receptor C3 subunit beta; ITGB2; integrin subunit beta 2; anti-CD18 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: MEM-48
• Specificity: The antibody MEM-48 recognizes an extracellular epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95kDa type I transmembrane protein expressed on all leukocytes.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD18 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: Alexa Fluor700
• Negative Species: Dog
• Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) Alexa Fluor 700 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human lymphocytes (red-filled) from human CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) Alexa Fluor 700 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD18 antibody

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the Functional Application (FUNC)tions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

References

*Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113(2):358-69., URL: http://www.ncbi.nlm.nih.gov/pubmed/18922855,*Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT: Increased phagocyte Fc gammaRI expression and improved Fc gamma-receptor-mediated phagocytosis after in vivo recombinant human interferon-gamma treatment of normal human subjects. Blood. 1997 Oct 15;90(8):3187-94., URL: http://www.ncbi.nlm.nih.gov/pubmed/9376602,*Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15;93(10):3505-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/10233903itool=,*Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95). J Immunol. 2000 Jun 1;164(11):5928-34., URL: http://www.ncbi.nlm.nih.gov/pubmed/10820275itool=,*Stefanova I, Horejsi V: Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules. J Immunol. 1991 Sep 1;147(5):1587-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/1715364dopt=A,*Larson RS, Springer TA: Structure and Functional Application (FUNC)tion of leukocyte integrins. Immunol Rev. 1990 Apr;114:181-217., URL:,*Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha). 1990;36(1):41-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/1971601dopt=A
*Dassanayake RP, Maheswaran SK, Srikumaran S: Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects. Infect Immun. 2007 Oct;75(10):5004-10., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Peters T, Sindrilaru A, Wang H, Oreshkova T, Renkl AC, Kess D, Scharffetter-Kochanek K: CD18 in monogenic and polygenic inflammatory processes of the skin. J Investig Dermatol Symp Proc. 2006 Sep;11(1):7-15., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Solovjov DA, Pluskota E, Plow EF: Distinct roles for the alpha and beta subunits in the Functional Application (FUNC)tions of integrin alphaMbeta2. J Biol Chem. 2005 Jan 14;280(2):1336-45., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Kess D, Peters T, Zamek J, Wickenhauser C, Tawadros S, Loser K, Varga G, Grabbe S, Nischt R, Sunderkötter C, Müller W, Krieg T, Scharffetter-Kochanek K: CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis. J Immunol. 2003 Dec 1;171(11):5697-706., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Shang XZ, Issekutz AC: Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol. 1998 Jun;28(6):1970-9., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=,*Drbal K, Angelisova P, Cerny J, Pavlistova D, Cebecauer M, Novak P, Horejsi V: Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun. 2000 Aug 28;275(2):295-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10964661dopt=,*Gao JX, Issekutz AC: Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers. Immunology. 1996 Jul;88(3):463-70., URL: https://www.ncbi.nlm.nih.gov/pubmedDb=pubmed&Cmd=

NCBI and Uniprot Product Information

• NCBI GI #: 89191865
• NCBI GeneID: 3689
• NCBI Accession #: NP_000202.2
• NCBI GenBank Nucleotide #: NM_000211.3
• UniProt Accession #: P05107; Q16418; Q53HS5; Q9UD72; B3KTS8; D3DSM1
• Molecular Weight: 84,782 Da
• NCBI Official Full Name: integrin beta-2
• NCBI Official Synonym Full Names: integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• NCBI Official Symbol: ITGB2
• NCBI Official Synonym Symbols: LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
• NCBI Protein Information: integrin beta-2; integrin beta chain, beta 2; complement receptor C3 beta-subunit; complement receptor C3 subunit beta; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; cell surface adhesion glycoproteins
• UniProt Protein Name: Integrin beta-2
• Protein Family: CD180 antigen
• UniProt Gene Name: ITGB2
• UniProt Synonym Gene Names: CD18; MFI7
• UniProt Entry Name: ITB2_HUMAN

NCBI Description

The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGB2: the integrin beta 2 subunit. Can combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Participates in cell adhesion as well as cell-surface mediated signaling. Defects are the cause of leukocyte adhesion deficiency type I (LAD1).

Protein type: Receptor, misc.; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; Cell surface

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cell surface; membrane; plasma membrane; integrin complex; receptor complex; vesicle; lipid raft

Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; cell adhesion molecule binding; ICAM-3 receptor activity; glycoprotein binding; protein kinase binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; apoptosis; cell-matrix adhesion; natural killer cell activation; receptor clustering; activation of NF-kappaB transcription factor; regulation of cell shape; leukocyte adhesion; cellular extravasation; cell-cell signaling; inflammatory response; cell adhesion; regulation of peptidyl-tyrosine phosphorylation; toll-like receptor 4 signaling pathway; aging; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of immune response; activated T cell proliferation; leukocyte migration during inflammatory response; heterotypic cell-cell adhesion; positive regulation of angiogenesis; toll-like receptor signaling pathway; endothelial cell migration; innate immune response; blood coagulation; leukocyte migration

Disease: Leukocyte Adhesion Deficiency, Type I